The aim of the present study was to evaluate the effect of culture media and disinfestation treatments on germination and the contamination in vitro of Zeyhera montana seeds. The trial was comprised of two experiments: in the first, the seeds were surface sterilized with alcohol 70% (1 min) + NaOCl 0.25, 0.5 or 1% active chlorine (5 or 10 min). The seeds were placed in flasks containing MS, MS rooting, Knudson B, Knudson C, Agar or WPM culture media. In the second experiment, the seeds were inoculated on the culture media which showed the best results in the previous experiment. Germination was slow and disuniform. The best results were 75% and 40% germination on Knudson C and MS rooting media, respectively
