AVIO L.

CAVALLINI A.

CELA F.

GIORDANI T.

INCROCCI L.

NATALI L.

PARDOSSI A.

SBRANA C.

SIMONI S.

TURRINI A.

VANGELISTI A.

English

Archivio della Ricerca - Università di Pisa

Proceedings of the LXV SIGA Annual CongressPiacenza, 6/9 September, 2022ISBN: 978-88-944843-3-5Oral Communication Abstract – 2.03 ARBUSCULAR SYMBIOSIS IN SOILLESS CULTURE: TRANSCRIPTOMIC ANDBIOCHEMICAL ANALYSES IN LACTUCA SATIVA PLANTS.VANGELISTI A.*, CELA F.*, SIMONI S.*, AVIO L.*, TURRINI A.*, SBRANA C.*,INCROCCI L.*, PARDOSSI A.*, NATALI L.*, CAVALLINI A.*, GIORDANI T.* *) University of Pisalettuce, symbiosis, transcriptomic, biofertilisier, soilless cultureLettuce (Lactuca sativa L.) is an annual autogamous diploid plant belongingto Asteraceae and amongst the top ten most profitable crop worldwide due toits nutritional value. Nevertheless, extensive cultivation of lettuce hasled to major problematics, especially in fertiliser exploitation both insoil and extra soil system, which represents a damage to the ecosystem andhas an increasing cost for the farmer and the final consumer. In thiscontext, arbuscular mycorrhizal (AM) fungi could help to reduce the impactof fertilisers improving plant nutrition, in particular for phosphorus (P)and nitrogen, contributing to grow healthier plants for human consumption.In our work, we compared lettuce plants (cv. Salinas) grown in soillessculture (peat-filled pots with subirrigation) with optimal P (phosphate)concentration (1 Mm) in the nutrient solution (+P/-AM), with under optimalphosphate nutrition (-P/-AM) and, finally, with plants grown with lower Pconcentration (0.5 Mm) and inoculated with the AM fungus Funneliformis mossae before transplanting (-P/+AM). Gene expression profile was analysedin both roots and leaves, and transcriptomic values were associated withphysiological and biochemical parameters determined at the end of theexperiments. Overall, in comparison with -P/-AM plants, 3,057 genes weredifferentially regulated by mycorrhizal symbiosis (1,505 in roots and 1,552in leaves) and 2,606 genes were induced by optimal phosphate nutrition (926in roots and 1,680 in leaves). Interestingly, the pattern of differentiallyexpressed genes showed major differences for -P/+AM plants, and suggestedan extensive regulation of genes related to photosynthesis, solutetransport (phosphate, nitrogen and sugar), metabolism of phytohormones(gibberellin, auxin), redox homeostasis, and transcriptional regulation(MYB, GRAS, WRKY, DELLA). Concerning metabolic pathways, -P/+AM plantsexhibited the activation of genes involved into production ofphenylpropanoids, carotenoids, and vitamins, whereas starch and sucrosemetabolism was similar in these plants and in the +P/-AM plants. These dataare coherent with the results of physiological and biochemical analyses(i.e. leaf area, dry biomass accumulation, leaf content of chlorophylls,total phenols and carotenoids). Finally, co-expression analysis allowed tofind a possible network, formed by 19 genes, which could explain thegreater growth rate observed in -P/+AM plants as compared with +P/-AMplants.  In conclusion, transcriptomic, physiological and biochemical datasuggest a possible exploitation of AM symbionts as biofertilisers forlettuce soilless cultivation with beneficial effects on both plant growthand leaf content of health promoting phytochemicals.

Arbuscular Symbyosis in soilless culture: transcriptomic and biochemical analyses in Lactuca sativa plants

https://arpi.unipi.it/bitstream/11568/1165473/2/Arbuscular_symbiosis_in_soilless_culture%20_transcriptomic_and_biochemical_analyses_in_Lactuca_sativa_plants..pdf

Arbuscular Symbyosis in soilless culture: transcriptomic and biochemical analyses in Lactuca sativa plants

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