Cazzola, Martina

Prenesti, Enrico

English

Institutional Research Information System University of Turin

18 October 2023AperTO - Archivio Istituzionale Open Access dell'Università di TorinoOriginal Citation:Polyphenols surface coating strongly reduce ROS/RONS production and selectively affect theviability of cancerous cellsTerms of use:Open Access(Article begins on next page)Anyone can freely access the full text of works made available as "Open Access". Works made availableunder a Creative Commons license can be used according to the terms and conditions of said license. Useof all other works requires consent of the right holder (author or publisher) if not exempted from copyrightprotection by the applicable law.Availability:This is the author's manuscriptThis version is available http://hdl.handle.net/2318/1677305 since 2018-09-26T11:46:12ZIntroductionPolyphenols are a structural class of natural-derived compounds characterized by the presence of multiples phenol groups.Each polyphenol has specific biological properties depending on the number and characteristics of phenol structures.In the last few decades polyphenols have been intensively studied because of their supposed capability to modulateinflammatory response to prevent cancerization and to promote tissue regeneration¹ and recently they have been suggested tofunctionalize the surface of currently used biomaterials to improve the final medical devices performance.The aim of the present work was to couple natural polyphenols extracted from green tea leaves to the surfaces of Ti6Al4V alloyand bioactive glass to promote functionality in healthy bone cells and death induced by DNA damage in cancer cells.Rita Sorrentino1, Sara Ferraris2, Martina Cazzola2, Andrea Cochis1, Barbara Azzimonti1, Lia Rimondini1, Enrico Prenesti3, Silvia Spriano2, Enrica Vernè2 1 Department of Health Sciences, Università del Piemonte Orientale UPO2 Department of Applied Science and Technology, Institute of Materials Physics and Engineering, Politecnico di Torino3 Department of Chemistry, Università degli Studi di Torinorita.sorrentino@med.uniupo.itPolyphenols surface coating strongly reduce ROS/RONS production and selectively affect the viability of cancerous cellsBioactive glass (CEL2) has been prepared bymelt and quenching technique² while Ti6Al4Vhas been chemically treated (Ti CT) to obtain anoxide surface with nanotextured morphologyenriched by hydroxyls groups³. Polyphenolswere extracted from green tea leaves (TPH) bysolvent extraction, freeze-dried, resuspended inwater and used to soak activated substrates.Finally, surfaces’ coating was chemicallyactivated by UV exposure. Surface analysiswas performed by means of Folin&Ciocalteutest, XPS and fluorescence analyses.Polyphenols selective antitumoral activity wasinvestigated on human osteosarcoma derivedosteoblasts (U2OS) in comparison with non-tumorigenic progenitor (human- fetal derivedosteoblasts – hFOB) by means of viabilityassay. The DNA damage and repair wereevaluated by nuclear immunolocalization of53BP1 and cyclin B1.REFERENCES1. S. Quideau, D. Deffieux, C. Douat-Casassus, L. Pouységu, Plant Polyphenols: Chemical Properties, Biological Activities, and Synthesis, Angew. Chem. Int. Ed. 50 (2011) 586- 621.2. S. Spriano, S. Ferraris, “Metallic Surfaces for Osteointegration” in Surface Tailoring of Inorganic Materials for Biomedical Applications, , L. Rimondini, CL Bianchi, E Vernè Eds,Bentham Science Publishers, 2012, pp 279-296 3. M N. Rahaman et al. Bioactive glass in tissue engineering, Acta Biomaterialia 7 (2011) 2355–23734. M. Cazzola, I. Corazzari, E. Prenesti, E. Bertone, E. Vernè, S. Ferraris, Bioactive glass coupling with natural polyphenols: Surface modification, bioactivity and anti-oxidant ability, Appl. Surf. Sci. 367 (2016) 237-248.The photometric Folin&Ciocalteu tests based on redox reactivitydemonstrated the effective grafting of bioactive and redox-activepolyphenols, while XPS and fluorescence analysis revealed thepresence of unmasked functional groups.Despite those preliminary results, polyphenol capability to selectivelyaffect cancer cells was appreciable only in coated bioglasses.As shown in figure 2a, the viability of bone cancer cell line (U2OS)was significantly affected in comparison with hFOB cells. Moreover,tumorigenic U2OS cells cytology revealed the presence of DNA-damage foci established by 53BP1 analysis. Nuclear localization ofcyclin B1 confirmed that the DNA-damage cannot be repaired (figure2b).On the contrary, despite an initial TPH induced cytotoxicity of U2OS,cancer cells viability increased during the analysis reaching controlsvalue (figure 2c). Additionally, the DNA damage and repair assaydidn’t show irreversible DNA damage for U2OS cells growth oncoated TiCT (figure 2d)CONCLUSIONSNatural-derived polyphenols were successfully grafted onto bioactive glass surfaces without losing polyphenol capability. Inparticular, polyphenols were able to selectively reduce cancer cells viability via ROS/RONS mediated DNA-damage and topreserve the viability and proliferation rate and of healthy osteoblastic progenitor cells.RESULTS AND DISCUSSIONMaterial and methods

Polyphenols surface coating strongly reduce ROS/RONS production and selectively affect the viability of cancerous cells

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Polyphenols surface coating strongly reduce ROS/RONS production and selectively affect the viability of cancerous cells

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