Additional file 1 of Leaf photosynthetic characteristics of waxy maize in response to different degrees of heat stress during grain filling

Abstract

Additional file 1: Figure S1. The conditions for temperature treatments. A: The photo of intelligent greenhouses; B: Average daytime and nighttime temperatures for each day during the gradient temperature treatment that lasted 15 days. Figure S2. Effect of HS during grain-filling stage on Chlorophyll fluorescence parameters of waxy maize leaves. A, B: ETR, apparent electron transfer rate; C, D: qP, photochemical quenching; E, F: NPQ, non-photochemical quenching; G, H: Fv/Fm, PSII primary maximum light energy use efficiency. T0, control; T1, mild HS; T2, moderate HS; T3, severe HS. Error bars denote standard errors from three replicates, and different letters at the same sampling date indicate significant difference at P <0.05. Figure S3. Effect of HS during grain-filling stage on soluble protein and sugar content of waxy maize leaves. A, B: soluble protein; C, D: soluble sugar. T0, control; T1, mild HS; T2, moderate HS; T3, severe HS. Error bars denote standard errors from three replicates, and different letters at the same sampling date indicate significant difference atP <0.05. Figure S4. Correlation analysis between waxy maize yield and various physiological indexes of leaves under different degrees of HS during grain filling. Chl, chlorophyll content; Pn, photosynthetic rate; Gs, stomatal conductance; Tr, transpiration rate; Ci, intercellular CO2 concentration; ETR, electron transfer rate; qP, photochemical quenching; NPQ, non-photochemical quenching; Fv/Fm, the photosystem II primary maximum light energy use efficiency; RuBPCase, ribulose bisphosphate carboxylase; PEPCase, phosphoenolpyruvate carboxylase; SP, soluble protein; SS, soluble sugar; MDA, malondialdehyde; ROS, reactive oxygen species; SOD, superoxide dismutase; POD, peroxidase; CAT, catalase; APX, ascorbate peroxidase; IAA, auxin; ABA, abscisic acid. Table S1. List of primer sequences used in this study