The effects of temperature, food concentration, and pH conditions on the life history characteristics of the freshwater rotifer, Brachionus calyciflorus, were investigated. The culture temperature (20, 25, and 30°C) had a significant relationship with life history parameters. At lower temperatures, there was a prolongation of the mean lifespan and juvenile period. The optimal temperature for the fecundity of this species was 25°C with an average quantity of 23.67±6.99 offspring female-1 across the entire lifespan of 6.7 days. No significant difference was found between the mean lifespan at different algae densities but the maximum fecundity (25.75±6.99 offspring female-1) was obtained at an algae density of 10x106 cells.ml-1. Brachionus calyciflorus could tolerate a broad range of pH (4-10) but preferred pH from 6-10. These results are critical for potential applications of this species in ecotoxicology, biomonitoring as well as in mass culture as live food for larval rearing in aquaculture