Impedimetric label-free immunosensor for rapid detection of Ochratoxin A in beer and wine

Abstract

In this work, the development of an impedimetric label-free immunosensor for the detection of Ochratoxin A in food products is reported. Two different immobilization schemes of a monoclonal antibody for OTA detection were investigated and compared, with the aim to develop an analytical device able to detect an OTA amount equal to the maximum levels imposed by European legislation for food products. activated ferrocene as electron-transferring mediator, improved the electrical properties of the immunosensor, which showed a limit of detection equal to 0.25 ng/mL. This immunosensor was used to analyze wine and beer samples spiked with known OTA amounts