Ali, AB

Kirchhecker, S

Thomson, A

English

UCL Discovery

Different classes of interneurones contribute differentially to network oscillations at distinctfrequencies. The pattern of transmission across excitatory synapses depends critically onboth the type of excitatory connection and the pattern of presynaptic activity. Existingknowledge of the properties of excitatory inputs to interneurones does not alone, however,explain the patterns of activity of particular subclasses of interneurones during specificnetwork oscillations in vivo (Klausberger et al., 2003). To begin to assess another criticalfactor, inhibitory inputs to interneurones were studied with paired whole cell recordings withbiocytin labelling and immunofluorescence in the CA1 region of rat hippocampus.1. IntroductionThis facilitation is in striking contrast to the depression typically seen at inhibitory inputs onto pyramidal cells from a wide variety of presynaptic interneurone classes and suggests that the delayed onset of inhibition in these interneurones may play an important role in their spike timing.Synaptic depression is typically seen at inhibitory inputs onto pyramidal cells from a widevariety of presynaptic interneurone classes and connections between S. pyramidaleinterneurones.3. “Depressing” interneurone IPSPs in S. Pyramidale-59mV50mV 1mV20msTrilamimar InterneuronePostsynaptic Pyramiddata from this pair published Ali et al EJN 1999Trilaminar interneurone presynaptic to a pyramid and a basket cell.5. Delayed onset of powerful facilitation at connections betweeninterneurones in S. radiatum.6. Reciprocal inhibitory connections in S. radiatum and S. Lacunosum moleculare, also exhibit powerful facilitation. This work was funded by the Medical Research Council (London) and Novartis Pharama(Basel)0.4mV-55mV0.05mV40mV100ms-55mV0.05mV40mV100ms-55mVSLM InterneuroneSR/SLM borderInterneuronePost in SR/SLM borderPre inSLM200ms-55mVSinglesweepSinglesweepPost in SLMPre in SR/SLMBorderProperties of unitary IPSPs elicited in lacunosum moleculare interneurones in the CA1 region of the rat hippocampus Ali AB, Kirchhecker S and Thomson, AMDepartment of Pharmacology, School of Pharmacy, 29/39 Brunswick Square, London, WC1N 1AX, UK2. MethodLocal circuit connections between interneurones were studied using dual whole-cellrecordings combined with double immunofluorescence and biocytin labelling in acuteslices of the CA1 region of 18-22 day old rat hippocampus.4. “Facilitating” IPSPs in S. lacunosum moleculare  50umAlveusSOSPSR  2mV20ms-69mVTrilamimar InterneuronePostsynaptic Basket CellThe IPSPs elicited in postsynaptic S. lacunosum moleculare interneurones by fast spiking interneurones located in S. lacunosum moleculare or S. radiatum, displayed strong paired pulse facilitation.7. Conclusion ReferenceAcknowledgments Klausberger, Magill, Marton, Roberts, Cobden, Buzsaki, Somogyi (2003) Brain-state- andcell-type-specific firing of hippocampal interneurons in vivo. Nature: 421(6925):844-8.Avidin-AMCAto label Biocytin FITC to Label CCKPostsynapticPresynapticPre                Post  SLMThere are several different times courses of facilitation observed betweeninterneurone connections in S. radiatum and S. lacunosum moleculare.Presynapticin SRPostsynapticin SLMTrains of presynaptic action potentials at short interspike intervals elicited IPSPs, which summated.Avidin-AMCA to label Biocytin+1.5nA-1.5nA100mV200ms20mV50 ms0.1mV-55mVPresynapticInterneuronePostsynapticInterneurone-70mV-70mV +0.5nA-1nA50mV200ms-60mV-70mV40mV50ms0.1mV-55mV40mV200ms+1.5nA-2.5nA+1.5nA-2nA-70mV-70mV0.05mV100 ms40mVPostsynapticin SLMPresynapticin SR/SLMBoarder-55mV0.05mV100 ms40mVPostsynapticin SLMPresynapticin SR/SLMBorder-55mV

Properties of unitary IPSPs elicited in lacunosum moleculare interneurones in the CA1 region of the rat hippocampus

https://discovery.ucl.ac.uk/10055070/1/afiaali_poster_Gordon%20conf%202005.pdf

Properties of unitary IPSPs elicited in lacunosum moleculare interneurones in the CA1 region of the rat hippocampus

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