DATA AVAILABILITY STATEMENT : All the data that support the findings of this study are available in the manuscript itself.Consumers are increasingly interested in purchasing meat products with low food additives content or even without the, since these kind of foodstuffs are perceived as more natural and healthier. Nitrites are the most commonly used additives in the meat industry to prevent the growth of pathogenic bacteria, keep red color (secondary effect on myoglobin = iron and oxygen-binding), and improve flavor. In this scenario, meat processors are challenged to produce nitrite-free products guaranteeing the microbial quality and sensory characteristics. The objective of the present study was to determine the effect of various natural extracts against the color of thermal processed beef, manufactured without nitrites. A total of fourteen natural alternatives have been evaluated: capsicum extract liquid phase (capsanthin), paprika oleoresin liquid phase, monascus yellow powder (Monascus purpureus), red yeast rice powder (Monascus purpureus) from three different producers, lycopene powder, red beet juice powder (Beta vulgaris), rosemary extract (Rosmarinus officinalis), capsicum extract powder (capsanthin), carmine pigment powder (cochineal extract), sorghum red pigment powder (Sorghum bicolor), and two factory-supplied recipes. For the first trial, extracts were added at a concentration of 0.3% in canned meat without nitrite. Samples were analyzed by colorimetric measurements before and after sterilization. The aim was to find natural extracts that provide similar color characteristics as canned meat with nitrite (used as reference). After color analysis, the extracts that did not show statistically significant differences (p > .05) from the positive control were chosen for the second trial, consisted of sample preparation at three different concentrations of extract (0,1%, 0,2%, and 0,3%) following factory manufacture procedures to ensure that the results were as accurate as possible. Results showed that sorghum red pigment powder (Sorghum bicolor) provides stable pigments and can be added as a natural additive to the manufacture of traditional canned meat recipe to maintain a similar red/pink color as same as provided by sodium nitrite.This research was funded by NoNit srl and a major food Processor, joint agreement 2020. Open Access Funding provided by Universita degli Studi di Perugia within the CRUI-CARE Agreement.http://wileyonlinelibrary.com/journal/jfpphj2023Paraclinical Science
