Additional file 5 of A novel CAR-T cell product targeting CD74 is an effective therapeutic approach in preclinical mantle cell lymphoma models

Abstract

Additional file 5: Figure S5. No significant depletion of circulating immune cells pre/post peak detection of 42105-74bbz CAR-T cells in a humanized mouse model. A Absolute cell numbers of B cells, monocytes, G-MDSC, M-MDSC and NK cells in humanized NSG mice on Day 3, 11 and 23 post UTT or 74bbz CAR-T engraftment. All human cells were identified by human CD45+. B: CD33−CD19+; Monocyte: LIN−CD45+CD11b+CD33+CD14+; G-MDSC: LIN−CD11b+CD33+CD14−HLA-DR−; M-MDSC: LIN−CD11b+CD33+CD14+HLA-DR−; NK: CD33−CD3−CD56+. Mice received either UTT cells (red, n = 5) and 42105-74bbz (blue, n = 7). Bars show the median cell number. B CD74 expression (blue) of B cells, monocytes, G-MDSC, M-MDSC and NK cells on Day 18 compared to isotype control (red). Data are from one mouse from the 42105-74bbz CAR-T cell treatment group