Negrini, K A

Shah, D

Sturgeon, K M

Wehrung, K

English

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           Role of Oncostatin M in Exercise-Induced Breast Cancer Prevention     Kara A. Negrini, Katherine Wehrung, Dhruvil Shah, Kathleen M. Sturgeon. Penn State College of Medicine, Hershey, PA  Epidemiologic studies and rodent models show moderate-intensity physical activity can decrease the risk of breast cancer. Proposed mechanisms of how physical activity impacts breast cancer progression range from minimizing risk factors to decreasing abnormal mammary cell proliferation. Muscle-derived cytokines, also known as myokines, are excreted by skeletal muscle following acute exercise. Specifically, the myokine oncostatin M (OSM), has been shown to decrease breast cancer cell proliferation in vitro. PURPOSE: To identify OSM involvement in physical activity-induced prevention of breast cancer in vivo. METHODS: Female, 22-day old, Sprague Dawley rats were injected with 50 mg/kg n-methyl-n-nitrosourea (MNU) to induce mammary adenocarcinoma. Rats were exercise (Ex) trained (MNU+Ex) or remained in standard cage conditions (Sedentary, Sed) (MNU+Sed). The study was powered with n=12 per group to observe a significant difference in tumor free survival time. Exercise training consisted of treadmill acclimation, and progressive increases in session duration, speed, and grade, until reaching 30 min/day, 20 m/min at 15% incline. Exercise training continued 5 days/week until tumor palpation or week 18, which ever came first. Rats completed a maximal endurance test (MET) after tumor palpation. Blood was drawn before, 30 min following, and 2 hr following MET to measure plasma OSM levels (pOSM). RESULTS: There were no significant differences between body weight growth curves of MNU+Sed and MNU+Ex groups during intervention. Tumor free survival was significantly higher (p=0.002; p<0.05) in MNU+Ex rats (52.2 ± 4.5 days post-MNU) compared to MNU+Sed rats (42.0 ± 0.0 days post-MNU). Following MET, pOSM levels were significantly higher compared to baseline pOSM levels in non-tumor bearing animals (p=0.046). Ongoing studies include analysis of pOSM in tumor-bearing animals after MET. MNU+anti-OSM+Ex groups were also added to observe changes in tumor latency after OSM blockade. CONCLUSION: Independent of differences in energy balance, exercise training increased tumor free survival in a rat model of carcinogen induced Mid Atlantic Regional Chapter of the American College of Sports Medicine  45th Annual Scientific Meeting, November 4th- 5th, 2022 Conference Proceedings International Journal of Exercise Science, Issue 9, Volume 11 breast cancer. The observed protection may be modulated by acute exercise-induced increases in OSM levels.    Supported by Penn State College of Medicine, Department of Comparative Medicine  

Role of Oncostatin M in Exercise - Induced Breast Cancer Prevention

https://digitalcommons.wku.edu/cgi/viewcontent.cgi?article=6674&context=ijesab

Role of Oncostatin M in Exercise - Induced Breast Cancer Prevention

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