Fifteen blood chemistry values for cotton rat (Sig-modon hispidur) were measured in 17 females ranged from 122.0 and 170.0 g and 11 males ranged from 91.0 to 190.0 g in weight. Significant sex differences were found for creatinine and triclyccridc (P &lt; 0.05). Six items (UA, TB, GPT, CK, HDLCHO, TG) in temales and 4 items (TB, GOT, GPT, CK) in males varied with coefficient of variation being over 40 %. The average values for TP, ALB, UA, TB in the cotton rat tended to be lower than those in the rat. On the contrary, the average values 0f GOT, GPT,&nbsp; ALP, CK, LDH, GLU tended to be higher than those in the rat

Ito, Tsunekata

Katahira, Kiyoaki

Ohwada, Kazua

English

Journals from University of Tartu

Scand. .1. [:Ih. Anim. Sci. N0. 1 . 1994 . V01. 21Reference values for blood chemistryin the cotton rat (Sigmadon hispidus)by Kazuo OhM'ada”, Tvunekala llol1and Kiyoaki Kazahiral)1’ Laboratory Animal Center, Yamagata University School ofMedicine,2-2-2. lida-nishi, Yamagata, 990—23 Japan.2) Experimental Animal Laboratory, Fukushima Medical College, 1 Hikarigaoka, Fukushima.960-12 Japan.IntroduczmnSince Armstrong (Armslrong 1939) used thecotton rat as a laboratory animal for the firsttime for studying poliomyelitis infection, cot-ton rats have been used for experimental fila-riasis studies (Bayer & Wonk 1983, Miller—Kehrmarm 1988, Pringle & King 1968. Ta-naka 1965. Tanaka 1967). This animal spe~cies is also used as an experimental host ofLeishmania donovani, Echinococcus spp.and paragonimiasis (lglauer el al. 1993,Vaux-Peretz 1991).The cotton rat (Sigmodan hispidus) belongsto the family Crecetidae in the suborderMyomorpha ofthe order Rodentia.We have previously reported hematologicalstandard va1ues (Katahira & Ohwada 1993)and body surface area (Chwada & Katahira1993) in this animal species.However, a few blood chemistry values re-mained undetermined. The reference valuesmust be established for quantitative use ofthecotton rat in research. Therefore, 15 refer-ence values for blood chemistry were recor-ded herein.Materials and methodsAnimals: Twenty—eight cotton rats (17 fema-les and 1 1 males) were bred in our laboratoryfor use in this study. AL Lhe time ofthe study,they ranged from 122.0 to 170.0 g (female)and 91.0 to 190.0 g (ma1e) in weight. The ani-mals were housed in plastic cages, lines withsterilized wood chips. These cages were keptTable 1. Reference values in blood chemistry for cotton rat.         . rwflfenale “aleItem(LniL) Abbreviation n 7 MegniSD Min.- Max. C.V. n McaniSD Min.- Max. C. 1'. Diff10151 prolein(a/01) Tp. 17 7.061: 0.34 6.40‘ 7.70 4.36 11 7.091‘ 0.62 6.20- 7.90 8.66 NSA133 in1R/dl) Alb. 17 2.95i 0.24 2.30— 3. 30 6. 22 11 3. 331 0.29 2 60- 3.50 9.27 NS‘ ea nitorogen1mz/dl) BUN 17 22.061: 3.34 17.40- 28.60 15.14 11 21.21L 2.53 17 80- 25.10 11.93 NS:ine(mL/c1) Crea. 15 0.651: 0.12 0.30— 1.00 27.33 11 0.501. 0.12 0 30- 0.70 23 66 P<0.05Uri: icid(ma/d1) UA 17 1.091: 0.75 0.40— 3.10 66.20 11 0.731: 0.28 0 40: 1.2 37.95 NS‘1irl!b1n(:ng/dl) TI). 17 0.13: 0.13 0.00- 0.40 70. 54 11 0.191: 0.19 0.00- 0.70 97.87 .\'SGlutamate oxaluace1a1s transaminase(1U/11 GOT 15 153.391; 23.71 113.60~214.20 16.72 10 120 41i56.20 41.60—202.40 46.68 VSG1utamate pyruvate lransaninase1lU/1) GPT 16 02.361: 33.96 35.30-164.70 40.93 11 63.951:34.39 11.40-113.80 53.76 NS.11ka11ne psosphataseW/l) AL? 17 123.45: 31.20 7070-15650 31.20 11 1391911982 69.60-191.10 21.42 N3Creatine p;osphokinase(lU/1) CK 17 236.16:13E.09 10070-62340 57.20 11 167.15:104.99 36. 70—35100 $2.61 NSLactate cehydragenase(IU/1) L0“ 17 260.00jf 73.67 164.50-446.50 28.41 11 271.46: 60.19 161.00-863.20 26.00 NSGlucose(1g/d1) G1u. 17 131.531: 65 97 53.70-352.00 34. 5 11 164.46: 35.44 107.20:237.20 21.55 NSCholestcrn1(1z/dl} Cho. 17 66.731: 22.95 32.90-131.40 34.39 11 60.211 20.04 56.30-121.00 24.96 NSHDL—Chalesler011mE/di) HDL-Chc. 17 34.961: 16.56 19.40- 81.30 47.35 11 42.93: 13.42 23.50- 67.20 31.26 NSTI]glyceIide(Jz/d1) TC 17 156.52i 69. 7 70.90-444.30 56.97 11 91.051: 30.61 53.50-136.7U 33.62 P<0.05Body weight1x) B.W. 17 140.292t 13.99 122.00-170.00 9.96 11 130.091: 26.33 91.00-150.GC 20.24 :SD:$1andard deviationC. V. :Coefficient of variationMin.~Max. :Mininum and maximum valueDiff. :Significant difference with sexNS No significant difference29Scand. J. Lab. Anim. Sci. V0. 1 . 1994 . V01. 21in an animal room at an ambient tempera-ture of 22 t 2‘C and 55 t 5% humidity.The animals were given a commercial soliddiet for rats (Charles River, Inc.) and waterad libitum with an autowatering apparatus.Blood collection: The animals were anesthe-tized with diethylether and exsangu-natedfrom the canal vena cava.Blood chemistry: Fifteen items. shown inTable 1, were measured. Measurements werecarried out with a VISIONTM system (DY-NABOTT). Whole blood was used for allanalyses and 2111 items were measured onthe day ofsamphng,Statistical analysis: A personal computer(NEC PC9801 UX) and statistical softwarepackage (VIEW FLEX Co. Ltd.) were used.The mean, standard deviations (SD) and co-eflieients of variation (C.V.) were calculated.The sex differences among items were alsoanalyzed.Results and dixcussionTable 1 shows the results expressed as themean and standard deviation, coefficient ofvariation, minimum—maxirnum value rangeaccording to sex.UA, TB, GPT, CK, HDL—CHO and TG va-lues in females and TB, GOT, GPT, CK va-lues in males varied widely with their coef—ficient of variation being over 40 %.Significant sex-related diITcrences were foundfor creatinine and triglyceride (P < 0.05).Compared to the reference values for rat (Mi-truka & Ruwmley 1991), the average valuesfor TP, ALB, UA= TB, TG in the cotton rattend to be lower than those in the rat. On thecontrary, the average values for BUN= GOT,GPT, ALP, CK, LDH, GLU in the cotton ratwere higher than those in the rat. These find-ings stimulate interest in comparative re—search to clarify the details.The range of fluctuation in measured valueswas small except for the items described inthe text.The present report provides useful referencevalues for the cotton rat.30SummaryFifteen blood chemistry values for cotton rat (SigLmodon hispidur) were measuredm 17 females ran-ged 1mm 1220 and 1700 g and 11 males rangedfrom 91.0 to 190.0 g in weight. Significant sex dif-ferences were found for creatinine and triclyccridc(P < 0.05). Six items (UA, TB, GPT, CK, HDL-CHO. T6) in temales and 4 items (TB, GOT.GPT CK)in males varied with coefficient ofvaria-tion being over 40 “/0 The average values for TP.ALB UA, TBin the cotton rat tended to be loweithan those in the rat. 0n the contrary, the averagevalues 01 GOT, GPT. ALP, (7K, LDH, GLU ten~ded to be higher than those in the rat.SammendragI den hiir rapporten publiceras referensvfirden f‘dr15 kemiska blodv‘zirden i béda ken av bomullsrét~tan (Sigmodon hispzdus).Yhzeemrez‘o /'K. PelkonenTéissé tutkimuksessa on mitattu 15 verimuuttujanviiteanot l7 naaraspuolibesta puuvillarotasta (cot—ton rat Sigmodan liispidus), joiden paino vaihteli91—190 gramman V'a'lillfi ja 11 uroksesta. joidenpaino vaihteli 91—190 gramman véililla Kreatinii-nin ja triglyseridien méiiréissfi oli merkittéivéisti eroasukupuolten vfilillé (p < 0.05). Vaihtelukertoin oliyli 40% naarailla kuudessa muuttujassa: virtsa-happo, kokonaisbilirubiini, GPT, kreatiinifosfoki»naasi, HDL—kolesteroli, triglyseridit, ja uroksillaneljéisséi muuttujassa: kokonaisbilirubiini, GOT,GPT, kreatiinifosfokinaasi. Kokonaisproteiini, a1»burniini, V’irtsahappo ja kokonaisbilirubiini olivatpuuvillarotalla alcmmat, mutta GOT, GPT, alka-linen fosfataasi. kreatiinifosfokinaasi, maitohappoja glukoosi korkeztmmat kuin rotalla.ReferencesArmrtmng C: The experimental transmission ofpolymyelitis to the eastern cotton rat, Sigma—don hispidus. Public Health Rep. 1939, 54.1719— 1721.Buyer M & P WenA. Homologus and cross react-ing immune response of the jird and cottonrat against microttliaria of Dipetalonema viteaeand litmosoides carinii (Nematoda: Filario—idea). Trop. Med. Parasit. 1983, 39, 3047308.Iglauer F, T Sc/mler, S Holub & R Sarhs: Ring—tail Disorder observed in cotton rats. Scand. JLab. Anim. Sci. 1993,20 Q), 119—12].Kamhira K & K Ohuuda; Hematological standardvalues in the cotton rat (Sigmodon hz'spidus).Exp. Anim. 1993, 42 (4), 653-656.Milmka BM & HA! Ranflrmle) Clinical biochemi-cal reference values In Clinical biochemicaland hematological reference valuesin normalexperimental animals and 1101ma1 humans, sc-cond edition. pp 153414, 1991, Masson pub-1ishing,USA.Mz‘illcr-Kchrmamz II: Antibody response againstLitomosoides Carini1 and the distribution ofbound antibodies on mierofirarie from the dif-ferent internal organs of cotton rats. Aeta.Tmp11988,45.361—372,()hwada K & K Kaluhira: Indirect measurementfor body surface area of cotton rats. Exp.Anim. 1993, 42 ('4). 635%37.Pringle G & DF King: Some developments intechniques for the study of the rodent tilaria1parasite. I. A preliminary comparison of thehost efficiency of the multimammate rat, prao-Seand .1 141111 Anim. Sci, Net 1 1 1994,1/0121mys (Mastomys) Natalensis, with that of thecotton rat, Slgmudun hispidus. Ann. Trop.Med.Parasit01, 1968, 62. 462—468.Tanaka H: Observation 011 the cotton rat as alaboratory animal for experimental filariasisstudies,Exp1Anim. 196114018046.Tanakn H & K Arai: Breeding ot‘cotton rat as thelaboratory animal. Exp. Anim. 1967, 1:6 (4},121—126.Vaux—Pprelz F & B Meignier: Lc rat des coton-niers Sigmndon hixpidus, Sci. Tech Anim.Lab. 1991,16,5—17.31

Reference values for blood chemistry in the cotton rat (Sigmadon hispidus)

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Reference values for blood chemistry in the cotton rat (Sigmadon hispidus)

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