A preliminary study has been conducted for the development of a bioprocess targeting the valorization of
orange peel waste (OPW). Essential oils and pectin were recovered from OPW through distillation, acid hydrolysis
and precipitation with ethanol. Optimal conditions for dilute-acid hydrolysis were investigated via estimation of the
sugars released and fermentation. Hydrolysis conditions of 109 o
C for 20 min and 116 o
C for 10 min using 5 % of
dry raw material produced the highest sugar yields at 0.76 and 0.77 (gtotal sugars gdry raw material-1) respectively. In order to
test the efficiency of enzyme hydrolysis as a pretreatment method for OPW valorization, cellulase production from
T. reesei was investigated, which was maximized following 5 days of cultivation. Furthermore, elemental analysis in
hydrolyzates from dilute-acid hydrolysis and a combination of acid and enzyme hydrolysis was performed. The
results indicate that during acid/enzyme hydrolysis, high concentrations of Mg2+ and Ca2+ ions are liberated in
contrast to dilute-acid hydrolysis. A. succinogenes fermentations of glucose and fructose were performed and the
succinic acid yields achieved were 0.66 (gsuccinic acid gglucose-1) and 0.33 (gsuccinic acid gfructose-1) respectively, while
galactose was not fermented. Overall, OPW may serve as a promising raw material for simultaneous production of
essential oils, pectin and succinic acid