Additional file 4 of Amyloid fibril proteomics of AD brains reveals modifiers of aggregation and toxicity

Abstract

Additional file 4: Table S2. List of proteins identified in label-free MS analysis of amyloid fibrils isolated from mouse cortices. Proteins identified with a higher abundance in purified fibrils (N = 8) obtained from AppNL-F/NL-F, and AppNL-G-F/NL-G-F, and 5xFAD mouse brains, 6 months age, compared to respective cortex homogenate as input (n = 3 - 4). Average NSAF values for purified fibrils and cortex homogenates for individual proteins were used. Each sheet represents individual mouse genotype, and each row has individual p values using Student’s t test and adjusted p values using Benjamini-Hochberg (BH) correction. Number of proteins with significantly higher levels in AppNL-F/NL-F, and AppNL-G-F/NL-G-F, and 5xFAD brains are 59, 32, and 29, respectively. Experiment = specific data set, Uniprot accession = Uniprot identifier for each protein, ratio = log2 average NSAF values (purified fibrils/homogenate), t test p value = t test p value, Rank = rank ordered proteins based on p value (if p values are identical, higher ratio was listed first). Additional remarks indicate if results (increased abundance in purified fibrils) are statistically significant or not