Historically, western societies have considered body image issues to predominantly affect young, White women. While in recent years men’s body image issues have been increasingly highlighted by researchers and the media alike, many instruments currently used to identify clinically significant body image disturbances were developed and validated with samples solely of women and/or girls. One such measure, Killen et al.’s (1994) Weight Concerns Scale (WCS), was initially validated in a sample of adolescent girls. The WCS has yet to be validated in samples of men, despite being used in large national surveys of college men and women (e.g., the Healthy Minds Study; HMS) used to inform resources on college campuses. Accordingly, we used structural equation modeling to conduct invariance testing between college student cisgender men’s (n = 2,248) and women’s (n = 4,733) responses on the WCS via the HMS. Through the use of two different approaches of invariance testing, evidence for metric noninvariance of two of the five items was identified, and all five items evidenced a response pattern that favored women over men. Additionally, removing noninvariant items on the WCS impacted the moderating effect of gender with indicators of depression, anxiety, and eating disorder symptomology. These findings suggest that the use of the WCS may not be appropriate for use in a cis-male sample without modification