F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
Abstract
Aims: Tumor-associated tissue eosinophilia
(TATE) has been correlated with prognosis in oral
squamous cell carcinoma (OSCC). This study aimed to
investigate whether eosinophils depletion affects
experimental oral carcinogenesis.
Methods and Results: BALB/c (wild type - WT) and
eosinophil-deficient (Δdb/GATA-1) mice were treated
with the carcinogen 4-nitroquinoline-1-oxide (4NQO) in
drinking water for 28 weeks. Tongues were collected for
histopathological and immunohistochemical analysis, as
well as for the evaluation of cytokines/chemokines by
ELISA. The tongue SCC induced by 4NQO was
associated with a rise in eosinophil numbers. WT-treated
group showed a significantly increased incidence of
SCC, with higher cytological atypia, in comparison with
Δdb/GATA-1 mice. Consistently, the proliferative index
was higher in WT compared to the Δdb/GATA-1/GATA1-treated
group. No significant changes in the
concentration of CCL3, CCL11 and TNF-α were
detected for both groups after 4NQO treatment.
Conclusions: These results suggest that eosinophils
might be responsible for the deleterious outcome of
experimental tongue carcinogenesis, given that their
ablation protects mice from OSCC