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Audrey P. Gasch

Camilla M. Kao

David Botstein

Gisela Storz

Michael B. Eisen

Orna Carmel-Harel

Pamela A. Silver

Patrick O. Brown

Paul T. Spellman

PubMed

We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing agent dithiothreitol, hyper- and hypo-osmotic shock, amino acid starva-tion, nitrogen source depletion, and progression into stationary phase. A large set of genes (; 900) showed a similar drastic response to almost all of these environmental changes. Additional features of the genomic responses were specialized for specific conditions. Promoter analysis and subsequent characterization of the responses of mutant strains implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators. Physio-logical themes in the genomic responses to specific environmental stresses provided insights into the effects of those stresses on the cell

Orna Carmel-harel

Patrick O. Brown*i

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Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes

http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.28.3788

Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes

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