The localization of fructose-1,6-
bisphosphatase (FBPase) in human skeletal muscle was
determined immunohistochemically using polyclonal
antibodies. Light microscopy analysis, confirmed with
the use of confocal microscopy, indicated that the
enzyme is localized on both sides of the Z line of
myocytes. The immunohistochemical investigation was
confirmed by a co-sedimentation experiment which
revealed that muscle FBPase binds strongly to a-actinin
- a major structural protein of the Z line. This is the first
report on localization of FBPase in skeletal muscle
tissue