Aim Bone defect is a challenge even for experienced orthopaedic
surgeons and it is a significant cause of morbidity in patients and a
source of high economic burden in health care. A severe bone defect is a condition whereby the bone tissue cannot undergo natural
healing despite surgical stabilization and requires further surgical
intervention. Stromal vascular fraction (SVF) is a heterogeneous
cell population derived from adipose tissue that results from minimal manipulation of the adipose tissue itself. TGF is essential
in maintaining and expanding mesenchymal stem cells or progenitors of osteoblasts. Furthermore, TGF-β signalling also triggers
osteoprogenitor cell proliferation, early differentiation, and maintenance of osteoblasts in the bone healing process. The aim of this
study was to determine the effect of administering SVF on bone
defects’ healing process assessed based on the TGF- β1.
Methods This was an animal study involving twelve Wistar strain
Rattus norvegicus. They were divided into three groups: negative
group (normal rats), positive group (rats with bone defect without
SVF application), and SVF group (rats with bone defect with SVF
application). After 30 days, the rats were sacrificed, the TGF- β1
biomarker was evaluated (quantified using ELISA).
Results TGF- β1 biomarker expressions were higher in the group
with SVF application than in the group without SVF application. All comparisons of the SVF group and positive control group
showed significant differences (p=0,000), respectively.
Conclusion Giving SVF application could aid the healing process
in a murine model with bone defect, marked by an increased level
of TGF- β1