Nuclear pore complexes (NPCs) are transport channels between the nucleus and the cytoplasm. The NPCs are composed by around 30 different proteins, termed nucleoporins (Nups) and each Nup is present in multiple copies. Recently, we and others discovered that several nucleoporins play critical roles during cell division including chromosome condensation, sister chromatid cohesion, kinetochore assembly and spindle formation. Nup58 is a part of the central transport channel of the NPC, which forms a complex protein with other nucleoporins such as Nup62 and Nup54. Recently, we showed that Nup62 plays a novel role in centrosome integrity. Here, we show that Nup62 interacts with Nup58 during cell mitosis. Next, we performed RNA interference-mediated knockdown of Nup58. Currently, we are investigating Nup58 depletion effect in cell cycle
