Additional file 1 of Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea

Abstract

Additional file: Figure S1. Construction of chimeric protein engineered strains of T. afroharzianum. (A) Sm1 and Chit42 overlap fragments for chimeric protein and TaSm1 and MaChit42 overexpression vectors construction; (B) PCR verification of chimeric protein and TaSm1 and MaChit42 engineered strains by using hygromycin primer; (C) and (D) were PCR verification of chimeric protein and TaSm1 and MaChit42 engineered strains using by differential primer pairs (PC between trpC promoter and Chit42; CS between Chi42 and Sm1; ST between Sm1 and trpC terminator; PS between trpC promoter and Sm1; SC between Sm1 and Chit42; CT between Chit42 and trpC terminator); (E) Southern blot analysis of chimeric protein and TaSm1 and MaChit42 engineered strains; (F) qPCR results of Sm1 gene expressing in T. afroharzianum with different culture medium (PDA and PD)