Electrokinetics has been used for guiding, pumping, and manipulating microfluidic particles for many years in the field of Biomedical, Microbiology, Chemistry, Medicine, and other fields of research, which makes it a ubiquitous tool for the multidisciplinary research on microfluidics. Between these two Alternating Current (AC) Electrokinetics have been proven to be more feasible for the researcher than the other one. In this research, we have investigated AC Electrokinetics and s effect on a new electrode configuration called V-electrode pattern. The V-electrode has been inspired and modified from the previous research work on the Orthogonal Electrode Pattern. In this research, this new electrode configuration has been analyzed using different types of setups, fabrication methods, and different fluidic conditions
