Not AvailableA hurdle technology-based approach for the reduction in immunogenicity
of shrimp major allergen, tropomyosin was investigated by combining electron beam
irradiation at 5 kGy and boiling, autoclaving, trypsin and chymotrypsin treatments
along with peeled raw shrimp as control. Shrimp extracts were prepared from the
irradiated sample and evaluated SDS PAGE profile, IgE binding ability and immunogenicity of tropomyosin using pooled sera of shrimp sensitive individuals. Electron
beam irradiation resulted in significant (p < 0.05) decrease in the IgE activity in the
case of all treatments. The SDS PAGE analysis showed the presence of tropomyosin
in all the treatments except autoclaving in combination with or without electron beam
irradiation. A pronounced reduction in allergenicity was observed in the treatment of autoclaving in combination with electron beam irradiation. The combined application of autoclaving and electron beam irradiation can be utilized for the preparation of hypoallergic shrimp.Not Availabl
