Effects of ozone therapy on acidic corneal burns in rats

Abstract

In the present study the effect of ozone therapy on hydrofluoric acid (HFA) related eye burnwas investigated in rats. A Total 20 healthy male Wistar albino rats (weighing 250 - 300 g withthe age of 16 weeks) were used. They were divided into groups (experimental and controlgroups) of 10 rats being housed individually and fed ad libitum. The HFA (2.00%) burn wascreated in all animals. The ozonized (20.00 µg O3 mL-1) bi-distilled water was applied as a drop(10.00 µL each drop) every 8 hr for 7 days in the experimental group. At the same time, 0.90%NaCl was applied as drop (10.00 µL each drop) every 8 hr for 7 days in the control group. In theexperimental group, intensive inflammation, angiogenesis, epithelial damage and stromaledema were detected in one animal. Epithelial vascularization and stromal edema were seen infour animals. In control group, only two animals’ corneal structures were normal. Inflammation,angiogenesis, epithelial damage, fibrosis, epithelial vascularization and stromal edema weredetected in the rest. As a result of this study, it was observed that local usage of ozone therapyhad a positive effect on the healing of corneal burns caused by HFA. It was concluded that moreozone-related studies should be done to enlighten the subject.&nbsp;</p