Trypanosoma spp. and Leishmania spp. are causal agents of a number of parasitic diseases. Culture media can be divided into 3 main categories: semisolid, biphasic, and liquid. While biphasic and semisolid culture media need blood, an important factor for the reproduction of parasites, most liquid media require fetal calf serum or erythrocyte lysate. A culture media RPMI-PY demonstrated a good performance in terms of time and parasitic load of L. infantum compared to other culture media. The aim of the work was to evaluate the performance of RPMI-PY medium in different Leishmania species and also to evaluate in T. cruzi culture. RPMI-PY is likely to be valuable additions to laboratory practice in light of the relatively simple recipes, general availability of the components, and in terms of suitability because rabbit breeding is not necessary and the costs are lowered and can be used for all Leishmania species and to cultivate T. cruzi
