At the beginning of the physiological growth of tobacco plants, there may be competition with weeds for light, moisture and soil nutrients, which slows down the development of the crop, raises management costs and in some cases, causes losses to the farmer. The objective of this work was to determine the persistence of weeds under chemical control before and after the transplantation of tobacco seedlings, for which a study was carried out in Campoalegre (Huila), at a height of 522 masl. A first sampling was carried out using the methodology by linear transects with 180 quadrants of 0.5 x 0.5 m, in 1.9 ha; later the application of herbicides was carried out and after the transplant a second sampling was implemented. Before transplantation, 16 botanical families with 23 species were identified and later 15 families with 19 species, without the presence of new records. Before transplanting, the highest index of importance value was of the Poaceae and the Cyperus rotundus species, but later the greatest value was presented again by the same family, although Rottboellia cochinchinensis stood out. Likewise, previously transplantation, the greatest diversity was of C. rotundus, dominance of Cleome spinosa and uniformity of Spermacoce assurgens. After transplanting, the greatest diversity and dominance was R. cochinchinensis and the greatest uniformity was Kallstroemia maxima. The survival capacity of the weeds is highlighted before a chemical control where only four species of those initially listed stopped appearing
