Integrative Analysis of Protein Interaction Data

Abstract

We have developed a method for the integrative analysis of protein interaction data. It comprises clustering, visualization and data integration components. The method is generally applicable for all sequenced organisms. Here, we describe in detail the combination of protein interaction data in the yeast Saccharomyces cerevisiae with the functional classification of all yeast proteins. We evaluate the utility of the method by comparison with experimental data and deduce hypotheses about the functional role of so far uncharacterized proteins. Further applications of the integrative analysis method are discussed. The method presented here is powerful and flexible. We show that it is capable of mining large-scale data sets. Introduction The knowledge of the whole genomic DNA sequence of organisms has started a new era in biological research. It is now for the first time possible to identify and analyze all genetic elements of a single organism, which are the Open Reading Fr..