Isolation of Candidate Genes Involved in Cold Temperatures Response in \u3cem\u3eFestuca Pratensis\u3c/em\u3e Huds., Using Suppression Subtractive Hybridisation and Microarray Approaches

Abstract

The objective of this work was to isolate candidate genes which are differentially expressed following cold-acclimation and develop SNPs to test for associations between candidate genes and frost tolerance. The ability to develop sufficient levels of tolerance against freezing temperatures through cold-acclimation (hardening) is crucial for survival of grasses and winter cereals in temperate climate. Meadow fescue (Festauca pratensis Huds.) is one of the most important forage grass species in Northern Europe. The preference of Festuca instead of Lolium in Norway is due to its superior combination of winter hardiness and forage quality

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