Aim: Metabolic disorders appear as risk factors for many diseases. One of the biggest causes of metabolic disorders is a high-fat diet. Increased dietary lipid with a high-fat diet increases lipogenesis and ectopic lipid accumulation, and therefore, increased peripheral insulin resistance leads to inflammatory response, chronic oxidative stress, and progressive organ damage. Exposure to cold induces adaptation to cold and provides adaptation with physiological changes. Bee venom treatment is performed with various methods such as apipuncture and direct injection of bee venom. In our study, the effects of bee venom and cold exposure on blood lipid levels in high-fat fed rats and whether they have synergistic effects were investigated.Methods: 3-week-old Spraque-Dawley male rats (30-40 g) were divided into 7 groups: control, cold stress (CS), high-fat diet (HFD), 0.5 mg/kg bee venom (BV), HFD+CS, HFD+ BV, HFD+CS+ BV. Rats were fed with high fat diet for 8 weeks. For the last 21 days, rats were exposed to cold for 2 hours a day and bee venom was injected subcutaneously for the last 15 days. Animals were sacrificed on the last day and fasting blood glucose was measured. Serum was then obtained, followed by lipid profile measurements. All data were presented as mean±standard error of the mean (SEM). Differences between the groups were assessed with One-way ANOVA Post hoc LSD test p<0.05 was considered significant.Results: Triglyceride levels were significantly lower in the HFD+ BV group compared to HFD group(p=0.024). HDL was significantly higher in HFD+BV(p<0.001) and HFD+BV+CS(p<0.001) compared to other groups. Fasting plasma glucose levels were significantly lower in HFD+BV(p=0.001) and HFD+BV+CS(p=0.007) compared to other groups.Conclusions: It has been shown in our study that exposure to cold and bee venom have a synergistic effect on blood lipid levels and fasting blood sugar disorders in a high-fat diet, and bee venom has a regulatory effect on triglyceride.</p
