Representative specimens of O. immunis and O. pellucidus were brought to the laboratory in order to investigate the influence of light on the occurrence of the symbionts encountered during the preliminary field study. After one week of acclimating the crayfishes they were distributed among environments control chambers that permitted exposure of the hosts and symbionts to 1) continuous light (C.L.), 2) a daily cycle of 12 hours of light and 12 hours of darkness (12L-12D) and 3) continuous darkness (C.D.)
