International Journal of Exercise Science 6(4) : 256-268, 2013. Previous research has considered the perceptions of athletes towards gender of coach and strength and conditioning coach. However, to date there appears little research considering the perceptions of clients towards gender of personal trainer. The purpose of this study was to investigate male and females perceptions of same- or opposite-gender personal trainers. Four hundred and two (male = 201, female = 201) undergraduate University student participants completed an adapted version of the Attitudes of Athletes toward Male versus Female Coaches Questionnaire (AAMFC-Q; 23). A 2 x 2 multivariate analysis of variance (MANOVA) revealed that neither males nor females showed any preference for gender of hypothetical personal trainer (p \u3e 0.05) although both males and females reported that a personal trainer of the opposite gender might make it harder to concentrate (p \u3c 0.05). Previous research has reported a preference for male coaches/strength and conditioning coaches from both male and female athletes. However, this study suggests that there is no such gender bias towards personal trainers in this population