International Journal of Exercise Science 15(1): 541-551, 2022. Variations of the deadlift can be executed using the hexagonal (hex) bar by altering, for instance, the knee and torso angles while maintaining a constant hip angle at the start position. PURPOSE: To examine muscle activation patterns of the biceps femoris, rectus femoris, and erector spinae during three deadlift variations using the hex bar. METHODS: Twenty resistance-trained male and female subjects performed hex bar deadlift variations in three different starting knee flexion positions: 128.4 ± 8.5°, 111.9 ± 8.7°, and 98.3 ± 6.5°. Subjects performed three repetitions at 75% of their three-repetition maximum. Electromyography sensors were placed on the dominant biceps femoris, rectus femoris, and lumbar erector spinae. A one-way repeated measures ANOVA was used to detect differences in mean and peak EMG values normalized to maximum voluntary isometric contraction (MVIC) (p \u3c 0.05). RESULTS: As knee flexion increased at the starting position, mean activation of the rectus femoris increased (24.7 ± 21.5 35.5 ± 25.4 62.1 ± 31.3% MVIC, p \u3c 0.001), while biceps femoris (40.6 ± 17.9 34.0 ± 16.4 28.1 ± 14.5% MVIC, p = 0.003) and erector spinae (73.0 ± 27.6 65.9 ± 34.4 54.9 ± 32.5% MVIC, p = 0.009) activation decreased. Peak activation of the rectus femoris increased (46.9 ± 33.0 60.9 ± 38.7 99.3 ± 41.6% MVIC, p \u3c 0.001) while decreasing in the erector spinae (118.6 ± 47.1 105.9 ± 49.4 89.1 ± 40.1% MVIC, p = 0.008). The rectus femoris experienced the greatest mean differences of the three muscles. CONCLUSIONS: Practitioners should consider the muscular goals when adjusting the starting position of a hex bar deadlift as posterior chain recruitment diminished and quadriceps activation increased as knee flexion increased