A new genus and species, Hispanobrya barrancoi Jordana and Baquero, gen. n., sp. n.
is described. It was found in the Gador Mountain range (Almer a, Spain). This genus is similar to
Capbrya Barra, 1999, from South Africa. The distinguishing characteristics of the new genus are the
presence of a post-antennal organ (PAO), no clear tenent hair, a characteristic unguis, the presence of
a reduced trochanteral organ, and flattened body setae. The distribution of the bothriotricha is similar
to that of the genus Capbrya

Baquero-Martin, E. (Enrique)

Christiansen, K. (Kenneth)

Jordana, R. (Rafael)

Martinez, M. (Maite)

Universidad de Navarra

A new genus and species of Entomobryidae (Collembola, Entomobryomorpha) from the Iberian peninsula

Dadun, University of Navarra

A NEW GENUS AND SPECIES OF ENTOMOBRYIDAE(COLLEMBOLA, ENTOMOBRYOMORPHA)FROM THE IBERIAN PENINSULAEnrique Baquero,2 Maite Martnez,2 Kenneth Christiansen,3 and Rafael Jordana2ABSTRACT: A new genus and species, Hispanobrya barrancoi Jordana and Baquero, gen. n., sp. n.is described. It was found in the Gador Mountain range (Almera, Spain). This genus is similar toCapbrya Barra, 1999, from South Africa. The distinguishing characteristics of the new genus are thepresence of a post-antennal organ (PAO), no clear tenent hair, a characteristic unguis, the presence ofa reduced trochanteral organ, and flattened body setae. The distribution of the bothriotricha is simi-lar to that of the genus Capbrya.KEY WORDS: Collembola, Entomobryomorpha, Entomobryidae, Hispanobrya gen. n., Spain,Iberian PeninsulaSpecimens of an undescribed collembolan with characteristics of both thefamilies Entomobryidae and Isotomidae were found during a study of the cavefauna of Almera (south of Iberian Peninsula). Their habitus, length of abdomi-nal segments III and IV, presence of PAO led to an initial identification asIsotomurus. Detailed study, including SEM (Scanning Electron Microscopy)observation, allowed us to see characters not visible under the light microscope.These details included the eye fine structure, claw, unguiculus, and body sculp-ture. As a result of this study it became clear that these specimens were a newgenus similar to the genus Capbrya Barra, 1999, from South Africa. METHODSThe specimens were collected in the ÒParaje Natural Karst en Yesos deSorbasÓ in Almera, Spain. The plant community of the ÒParaje Natural Karst enYesos de SorbasÓ is gypsophilous and is dominated by small bushes of Thymusspp. (thyme, Lamiaceae). There are few other plants near these. In the more openregions, where the thyme is less dense, there is a scattering of annual plants (L-zaro, 1986). The remaining soil is covered mainly by lichens, which cover about90 percent of the gypsum surface. The predominant lichen community is Helian-temo alypoidis-Gypsophiletum struthii (Rivas-Goday and Esteve, 1965).Samples were taken in the spring, summer and autumn, but Hispanobrya gen.n. was found only in the spring and summer. Specimens were preserved in 70percent ethanol. Some specimens were mounted on slides using ÔHoyer medi-um,Õ whereas others were dehydrated using an ethanol series followed by criti-cal-point drying in CO2, mounted on aluminum SEM stubs, and coated in ArgonVol. 115, No. 4, September & October 2004 229______________________________1 Received on August 4, 2004.  Accepted on October 20, 2004.2 Department of Zoology and Exology, University of Navarra, P. O. Box 177, 31080 Pamplona,Navarra, Spain. Corresponding author: Enrique Baquero, ebaquero@unav.es. Mate Martinez,mmartin4@alumni.unav.es; Rafael Jordana, rjordana@unav.es.3 Grinnel college, Grinnell, Iowa 50112 U.S.A. christak@grinnell.edu.Mailed on February 3, 2005abdominal segments III and IV posterior and in front of the last row of setae, and in abdominal seg-ment V with the anterior sensillae three times as long as the posterior. Pseudopores on thoracic andabdominal segments I-IV. Bothriotricha on abdominal segments II-IV (2/3/2) (Fig. 2C,4A). There are3+3 pubescent setae (type 2 from Christiansen, 1958) on abdominal segment IV similar to the both-riotricha but thicker. Macrosetae similar to the Entomobrya type 1 setae occur on the thorax andabdomen but are somewhat different in appearance from those seen in the genus Entomobrya (Fig.1D,3L). The rest of setae are more flattened with 6-8 rows of barbed ridges; the basal barbs projectout laterally more than the distance of the seta width. This type of setae are 19-46 micra long.Macrosetae present on head, thoracic segments and abdominal segment I (Fig. 2A,4A). Mesosetae onabdominal segments II-VI (Fig. 2B) and on legs up to 25 micra long. Trochanteral organ on leg IIIhas four smooth setae (Fig. 3D,E). On the leg III there is a smooth and long inner seta. Pretarsus witha single anterior seta. The unguis (Fig. 3F-J) with three longitudinal ridges extended beyond the levelof the basal teeth, with the central ridge longer than the others, inner lamellae joined basal to apexforming the apical tooth. Between the basal paired teeth and the anteapical teeth there is an unpairedVol. 115, No. 4, September & October 2004 231atmosphere with 16 nm of gold in an Emitech K550 sputter-coater. SEM obser-vations were made in a Zeiss DSM 940 A. Abbreviations: MZNA, Museum ofZoology, University of Navarra.In addition to direct capture, specimens were captured with two types of pit-fall traps. One, containing a solution of water and chloral hydrate (10g/l), wascollected after 24 hours. A second type had a mesh and 200 g of sheep/goatmanure without preservative. The specimens in these traps were collected direct-ly after 48 hours, with an ethanol wetted paintbrush.Hispanobrya Jordana and Baquero, NEW GENUSDiagnosis: Body without scales. Abdominal tergite IV 1.72 times as long asabdominal tergite III (1.6-1.9; n=6). First antennal segment undivided, fourthantennal segment with apical vesicle but without a laminar projection. 7+7ommatidia with reticulated cornea, and a very poor developed 8th ommatidium.Its cornea are almost invisible with the SEM, but the ommatidium lens is barelyvisible under light microscopy. The PAO is in the form of a protruding vesiclewith a perforated cavity on top (Fig. 1A). There are no clear tenent hairs on legs.A reduced trochanteral organ is present in leg III. The formula of trichobothria is2, 3, 2 on abdominal tergites II, III and IV.Type species. Hispanobrya barrancoi sp. n. Jordana and Baquero.Etymology. The generic name refers to the geographical region (Hispania,Latin for the Iberian Peninsula).Hispanobrya barrancoi Jordana and Baquero, NEW SPECIES(Figs. 1-4)Description. Total length without appendages 0.7-1.0 mm (n=20) (holotype, 0.95 mm) (Fig. 1B).Background color whitish, with some pigment on head and antennae, and bands covering most of thetergites of all segments. Under SEM the body presents a very uniform reticular pattern, consisting oflarge hexagonal cells with primary tubercles at the corners (Fig. 1A,C).Antennae longer than head (holotype antenna/head ratio 2.2). Length of antennae segments (holo-type): 0.07:0.11:0.12:0.14 (Fig. 3A,B). Antennal segments II and III similar in length. Antenna withabundant sensillae (about 50) of three types: 1) short, cylindrical, blunt and smooth; 2) long (twotimes the preceding) and smooth (Fig. 1C); 3) longer and serrated asymmetrically. Antennae segmentIV with extrusible apical vesicle but without laminar projection. Antennae segment III with fewersensory setae than segment IV, and an apical organ with two leaf-like perforate sensory organs (Fig.3C). Antennal segment II with fewer sensillae than III. Antennal segment I with two basal sensillaeon dorsal side, and five basal sensillae on ventral side.Head. The PAO is a protruding vesicle (20 x 10 micra in a Paratype) with a perforated cavity ontop (11.5 x 6.9 micra), situated in front of the eyes A and B. Under SEM the cavity of the PAO showsperforations (Fig. 1A), and 7+7 reticulated ommatidia can be observed. The G ommatidia cannot beseen under the SEM, but a small lens can be seen under the light microscope. A large number ofmacrosetae of different sizes present (Fig. 4B). The bothriotrichum beside the eyes is present.Labrum with 554 setae (Fig. 4C). Labral papillae smooth with an anterior row of short blunt setae.The remaining labral setae are smooth and acuminate, the five prelabrals setae ciliated. The labialsetae drawn appear like a typical entomobryid labial triangle with the M1, M2, R, E, L1 and L2 setae,all similar and ciliate, and seta a2 smooth present (Fig. 4B). Labial palps with five basal setae (Fig.4D); the external differentiated papilla has a blunt, thickened seta. Venter of head with mesosetae ofvaried sizes. Body chaetotaxy. Microsensillae: 10/1000. Sensillae: 12/02223, thoracic ones lateral but those of230 ENTOMOLOGICAL NEWSFig. 1. Hispanobrya barrancoi (SEM microphotographs). A, Detail of the eyes and PAO(bar = 10 micra). B, Lateral habitus (bar = 100 micra). C, Two types of sensillae presenton the antennal segment IV, one of them smooth and the other with peculiar spine-likeprojections, very difficult to see under light microscope (bar = 2 micra). D, Mesosetae anddetail (x4) (bar = 5 micra). Vol. 115, No. 4, September & October 2004 233232 ENTOMOLOGICAL NEWSFig. 3. Hispanobrya barrancoi. A, Antennae dorsal view. B, Antennae ventral view.C, Detail of the sensory organ of antennal segment III. D, Leg III. E, Trochanteral organ.F-J, Claw rotation to show its structure. K, Ventral tube (collophore). The arrow points tothe head. L, Detail of a mesosetae (type IV, Christiansen, 1958) from the body.M, Tenaculum. N, Furcula.tooth. The unguiculus is half as long as the unguis, and has two short lateral wings but no terminalfilament. The four lamellae have a strong ridge. The lateral flaps of the ventral tube each have eightapical setae, only one being smooth, anteriorly with 1+1 setae, and no posterior setae (Fig. 3K).Tenaculum with four teeth on each ramus, and a single seta on the corpus (Fig. 3M).Furca. Manubrium with setae similar to those on the legs, manubrial plaque with two pseudo-pores, two inner setae and one outer seta (Fig. 3N). Dentes ringed on its basal two thirds (Fig. 2D),with the final third narrowed and striated transversally, and with three subapical setae that reach theapex of the mucro, which is falciform and without basal spine.Type Material. Holotype. Female, SPAIN, ÒParaje Natural Karst en Yesos de Sorbas,Ó Almera,300 m (UTM co-ordinates 30SWG8308), Mediterranean maquia, 28 June 2002, Ruiz-Portero (pitfalltrap) code: MZAL0164-02p (slide) (MZNA). Paratypes. Same data as for holotype, MZAL0164-02(6 specimens in ethyl alcohol), MZAL0164-04p (slide) and MZAL0164-03, 05, 06 (3 specimens on2 SEM stubs) (MZNA).Other material studied. Same locality as for holotype, MZAL0150-03p (1 specimen on slide), 9August 2002 (direct capture and pitfall trap); MZAL0151-02t (1 specimen in ethyl alcohol), 7 AugustFig. 2. Hispanobrya barrancoi (SEM microphotographs). A, Macrosetae on the thoracicsegment II (bar = 10 micra). B, Lateral view of abdominal segment IV (bar = 30 micra).C, Bothriotrichum on abdominal segment II. (bar = 5 micra). D, Lateral view of the fur-cula and detail of the mucro (inset x10) (bar = 20 micra). The presence of PAO of Hispanobrya Jordana and Baquero, gen. n., Capbryaand Nothobrya is similar to Indoscopus Prabhoo, 1971, at least one species ofAlloscopus Brner, 1906 and Australotomurus Stach, 1947; however, the otherfeatures of Hispanobrya are totally unlike those found in these genera. The PAOis also found in some genera of Tomocerinae. The presence of type 1 setae, thetrochanteral organ on leg III and the 2- 3- 2 arrangement of bothriotricha onabdominal segments II-IV, as well as the habitus indicate that all three generabelong in the family Entomobryidae sensu lato, but the chaetotaxy, the detailedstructure of the setae and the unusual features of the unguis in Hispanobrya doesnot fit into any subfamily as they are presently defined. These discoveries raise two important questions: 1) what does the discoveryof these two genera in two such disparate localities imply and 2) what is theimpact of this upon the suprageneric classification of the Entomobryidae sensulato.ACKNOWLEDGEMENTSWe are grateful to Dr. Pablo Barranco (Department of Applied Biology, University of Almera)for the material object of the study. Stephanie Peterson assisted in the preparation of the manuscript. LITERATURE CITEDBaquero, E., R. Jordana, and K. Christiansen. 2004. Redescription of Nothobrya schubarti Arl,1961 (Collembola, Entomobryomorpha). Entomological News 115: 31-34.Barra, J.-A. 1999. Un nouveau genre Capbrya avec deux nouvelles espces de la Province du Cap(Rp. Sud Africaine) (Collembola: Entomobryidae). Bulletin de lÕInstitut Royal Des SciencesNaturelles de Belgique 69: 19-24.Christiansen, K. A. 1958. The Nearctic members of the genus Entomobrya (Collembola). Bulletinof the Museum of Comparative Zoology, Harvard 118: 440-545.Lzaro, R. 1986. Sobre la flora y vegetacin gipscola almeriense (Fanergamas). Boletn delInstituto de Estudios Almerienses 6: 131-150. Rivas-Goday, S. and F. Esteve. 1965. Nuevas comunidades de tomillares del sudeste rido ibri-co. Anales del Instituto Botnico AJ Cavanilles 23: 7-78.Vol. 115, No. 4, September & October 2004 2352002, (direct capture and pitfall trap); MZAL0152-02t (1 specimen in ethyl alcohol), 14 June 2002(direct capture and pitfall trap); MZAL0153-03t (1 specimen in ethyl alcohol), 26 June 2002 (directcapture and pitfall trap); MZAL0154-03t (1 specimen in ethyl alcohol), 17 May 2002 (direct captureand pitfall trap); MZAL0161-03t (2 specimens in ethyl alcohol), 31 May 2002 (direct capture and pit-fall trap); MZAL0162-03t (4 specimens in ethyl alcohol), 27 August 2002 (direct capture);MZAL0163-03t (1 specimen in ethylalcohol), 6 May 2002 (direct captureand pitfall trap) Ruiz-Portero (allMZNA).Etymology. The name isdedicated to Dr. Pablo Barran-co, who kindly provided thematerial.DISCUSSIONCapbrya Barra from SouthAfrica is the genus most simi-lar to Hispanobrya. Hispano-brya is similar to Capbrya inhabitus, the falciform mucro,the dentes without spines,crenulated with the final thirdstriated transversally, the num-ber and position of the bothri-otricha and type 2 setae onabdominal segment IV, theratio between abdominal ter-gites III and IV, the generalchaetotaxy and absence of aclear tenent hair. It differs inclaw morphology, the sensoryorgan on antennal segment III,the eye number, and the PAO.The lack of SEM figures forthe antennae of Capbryamakes comparison of the sen-sory setae difficult.Nothobrya Arl, 1961 issimilar in size and habitus, buthas a vesicular PAO and sixsegmented antennae (Baqueroet al., 2004). The setae mor-phology and distribution arealso different.234 ENTOMOLOGICAL NEWSFig. 4. Hispanobrya barrancoi. A, Body dorsalchaetotaxy. B, Dorsal (a) and ventral (b) headchaetotaxy. C, Labrum. D, Labial papillae.

A new genus and species of Entomobryidae (Collembola, Entomobryomorpha) from the Iberian peninsula

Abstract

Similar works

Full text

Available Versions

Universidad de Navarra

Dadun, University of Navarra