Biocompatibility of electrospun polymer nanofibers (EPNs) with NIH 3T3 cell line and interaction of quercetin with different flavonoids: Induction of phase II enzymes in vitro cancer cell lines

Abstract

Part I: In this study Electrospun nanofibers, Elasthane™ 55D, Elasthane™ 75D, Biospan®, Hydrothane™ and Lycra® were evaluated for their biocompatibility to be artificial tissue substitutes using NIH-3T3 fibroblast cells. A colorimetric based MTT dye reduction assay demonstrated that EPNs did not stop cell growth in vitro. Additionally, fluorescence confocal microscope images verified that cell adhesion occurred in the nanofibers, and cell growth on the EPNs over two weeks period was confirmed by DNA quantification. Part II: This section of the thesis is focused on evaluation of chemopreventive properties of various phytochemicals by induction of Phase II enzymes. The interaction of Quercetin at an optimum concentration in combination with different flavonoids (Hesperedin, Ginger, Resveratrol and β-Carotene), were used to determine the induction of Phase II enzymes in vitro cancer cell lines; caffeine was incorporated in the experimental groups to assess its protective properties on the induction of phase-II enzymes