Additional file 1 of Attenuation of OX40 signaling suppression by age disrupts peripheral deletion of CD4+ T cells specific for the epidermal autoantigen desmoglein 3

Abstract

Additional file 1: Table S1. A comprehensive list of antibodies used in the experiments as well as information on the target antigen, clone, type of fluorescence, catalog number, and the respective supplying companies. Fig. S1. a,Outline of the adoptive transfer of Dsg3H1-Rag2−/− T cells and WT CD4+ T cells to Dsg3−/− or WT mice. b, FCM plots of Dsg3−/−and WT mice after the transfer of CFSE-labeled Ly5.1+ Dsg3H1-Rag2−/− T cells and Ly5.2+ WT T cells. The gating strategy used to identify 7AAD− CD4+ cells is illustrated. Ly5.1+Dsg3H1-Rag2−/− T cells and CFSE+Ly5.1− co-transferred WT T cells were gated (red and black squares, respectively). Proliferation indicated by CFSE intensity reduction of Ly5.1+Dsg3H1-Rag2−/− T cells are observed only in WT mice. Fig. S2. a, FCM plots demonstrate the gating strategy employed to identify proliferating Ly5.1+ Dsg3H1-Rag2−/− Tcells in SLN. Cutoff values for the expression of IFN-γ, Birc5, and OX40 in Dsg3H1-Rag2−/− T cells were based on their expression levels in recipient T cells. Fluorescence minus one (FMO) controls of CD4, Ly5.1,CFSE, IFN-γ, Birc5, and OX40 are also shown. b, FCM plots show the gating strategy employed to identify four subsets of migrating dendritic cells in SLN. FMO controls of CD45, CD11c, IA/IE, CD11b, Langerin and CD103 are also shown. c, FCM plots demonstrate the gating strategy employed to identify Tregs in SLN. FMO controls of Foxp3 and OX40 are also shown