Erbium-YSGG laser systems are promising tools in ear, nose and throat (ENT) surgery. The high absorption in biological tissues, resulting in precise tissue ablation with minimal thermal tissue damage, and the possibility to guide the radiation through optical fibres make the 2.79 Μm wavelength a favourite for microsurgery. In order to simulate the fenestration of the human stapes foot plate required for prosthesis implantation when treating otosclerosis, five guinea pig cochleae were irradiated in vivo until perforation was achieved. The laser-induced temperature rise and pressure transients evoke activity in the inner hair cells that was investigated by micro-iontophoresis. Perforation of the cochlea bone (hole diameter of 350 Μm) can be performed with a few laser pulses and high precision with a thermal damage zone of<100 Μm. The bone ablation rate is 10 ± 2 Μm pulse-1 at a radiant exposure of 12 J cm-2. The functionality of the afferent inner hair cells in the guinea pig cochlea was verified before and after laser treatment using glutamate receptor agonists AMPA and NMDA. For the above selected laser parameters, the induced 15-min enhanced activity was blockable with the specific reversible AMPA and NMDA antagonists CNQX and AP-7. Micro-iontophoresis confirms the reversibility of cochlea functionality after its perforation with Er-YSGG laser pulses. A limit of radiant exposure around 12 J cm-2 is found for safe fenestration. It is demonstrated that the Er-YSGG laser is a precise and safe instrument whilst still using adequate laser parameters. On the other hand, this study demonstrates the potential of uncontrollable and unintended induced damage, resulting from vapour channel formation in the perilymph, if a high laser radiant exposure is applie
