Effects of electron acceptors on CH4 emission in alpine wetlands

Abstract

Alpine wetlands are an important source of methane (CH4) and play a key role in the global carbon cycle. Their CH4 emissions largely depend on microbial CH4 production and oxidation processes that involve external electron acceptors. Seasonal precipitation drives redox cycles of humic acids (HAs), iron oxide and sulfur species, which will in turn affect CH4 production and oxidation. To investigate the effects of electron acceptors on CH4 emissions, soil samples from a typical alpine wetland on the Tibetan Plateau were incubated with the addition of ferrihydrite (HFO), HAs, sodium sulfate (SO42-) or combinations (HAs-HFO, HAs-SO42- and HAs-HFO-SO42-). During long-term anaerobic incubation, CH4 concentrations showed similar trends, increasing rapidly from 0 to 60 days, decreasing from 60 to 240 days, and finally slowly increasing again after 240 days, in all treatments except the sterilised control. Thus, the incubation period was divided into the production, consumption and reproduction phases. The addition of HFO, HAs or HAs-containing electron acceptors promoted the rates of both production and consumption of CH4, increasing the production potential of CH4 by 65–100 % and the oxidation potential of CH4 by 58–115 %. On the other hand, SO42- inhibited the production and consumption of CH4, reducing the production potential by 35 % and the oxidation potential by 50 %. Electron acceptors such as HFO, HAs and SO42- play important roles in CH4 emissions. HAs are the dominant factor affecting CH4 emissions in alpine wetlands. From a broader ecological perspective, organic and inorganic electron acceptors play a key role in CH4 production and oxidation under anaerobic conditions, influencing CH4 emissions from alpine wetlands