Two novel reversible enzyme inhibitors involved in monoamine metabolism are described. The novel and reversible inhibitors are the catechol-O-methyl-transferase (COMT) inhibitor, Ro 40-7592 (3,4-dihydroxy-4'-methyl-5-nitrobenzophenone), and the monoamine oxidase type-B (MAO-B) inhibitor, Ro 19-6327 (N-(2-aminoethyl)-5-chloro-2-pyridine carboxamide HC1). These may be of special therapeutic benefit in Parkinson's and Alzheimer's disease

Da Prada, M.

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THE CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES New Approaches to the Treatment of Age Related Brain Disorders M. Da Prada ABSTRACT: Two novel reversible enzyme inhibitors involved in monoamine metabolism are described. The novel and reversible inhibitors are the catechol-O-methyl-transferase (COMT) inhibitor, Ro 40-7592 (3,4-dihydroxy-4'-methyl-5-nitrobenzophenone), and the monoamine oxidase type-B (MAO-B) inhibitor, Ro 19-6327 (N-(2-aminoethyl)-5-chloro-2-pyridine carboxamide HCI). These may be of special therapeutic benefit in Parkinson's and Alzheimer's diseases. RESUME: Nouvelles approches du traitement des affections du cerveau reliees au vieillissement. Nous decrivons deux nouveaux inhibiteurs enzymatiques a action reversible impliques dans le metabolisme monoaminergique. Ces nouveaux inhibiteurs dont Taction est reversible, sont des inhibiteurs de la catechol-O-methyl-transferase (COMT), le Ro 40-7592 (3,4-dihydroxy-4'-methyl-5-nitrobenzophenone) et l'inhibiteur de la monoamine oxydase de type-B (MAO-B), Ro 19-6327 (N-(2-amino-ethyl)-5-chloro-2-pyridine carboxamide HCI). Ces produits pourraient s'averer particulierement benefiques dans le traitement de la maladie de Parkinson et de la maladie d'Alzheimer. Can. J. Neurol. Sci. 1991; 18: 384-386 This survey deals with the main neurochemical characteris-tics of two novel reversible enzyme inhibitors involved in monoamine metabolism and the rationale for their therapeutic use in age-related neurodegenerative brain disorders, in particu-lar Parkinson's disease (PD) and Alzheimer's disease (AD). The novel and reversible inhibitors are the catechol-O-methyl-trans-ferase (COMT) inhibitor, Ro 40-7592 (3,4-dihydroxy-4'-methyl-5-nitrobenzophenone, and the monoamine oxidase type-B (MAO-B) inhibitor, Ro 19-6327 (N-(2-aminoethyl)-5-chloro-2-pyrdine carboxamide HCI). Chemical structure ofRo 40-7592 (left) and Ro 19-6327 (right) Novel Strategies for the Treatment of Parkinson's Disease For the symptomatic treatment of PD coadministration of the COMT inhibitor Ro 40-7592 with Madopar® is proposed to optimize the neurotransmitter replacement therapy with 3,4-dihydroxy-phenyl-L-alanine (DOPA). The potent COMT inhibitor Ro 40-7592, by blocking the COMT activity intra-and extra-cerebrally should markedly improve the pharmacokinetics and pharmacodynamics of DOPA. Recent trials in healthy sub-jects provided a first clear evidence that Ro 40-7592 (200 mg) given in combination with Madopar®-125 standard markedly enhances the bioavailability of DOPA in plasma. These new findings also allow us to anticipate that the pharmacokinetics of DOPA will be further optimized with a treatment which com-bined Ro 40-7592 with the controlled release formulation Madopar® HBS.1 Alternatively, the highly selective MAO-B inhibitor Ro 19-6327 given with Madopar® might be useful in the therapy of PD because Ro 19-6327, like the irreversible MAO-B inhibitor selegiline (1-deprenyl), should improve dopamine (DA) neuro-transmission by blocking the beakdown of DA in the nigrostri-atal dopaminergic nerve terminals2'3 or adjacent cells. Recently it was suggested that exogenous neurotoxins could play a criti-cal role in the process of neuronal degeneration that character-izes PD. For instance, inhibition of MAO-B with selegiline4 or Ro 19-63275 dramatically reduces the conversion of l-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) into its dopaminer-gic neuroxtoxin l-methyl-4-phenylpyridinium ion (MPP+). The discovery that a relatively simple compound, such as MPTP, produces Parkinson-like features in humans and animals and the fact that MAO-B inhibitors counteract MPTP-induced neurotox-icity in experimental animals raised the suspicion that environ-mental toxins might cause PD.6 Most important, several clinical studies indicate that the MAO-B inhibitor selegiline might protect against neurodegenerative diseases of the aging brain, e.g. in From the Pharma Research Department, F. Hoffmann-La Roche Ltd., Basel, Switzerland Reprint requests to: M. Da Prada, Pharma Research Department, F. Hoffmann-La Roche Ltd., Ch-4002 Basel, Switzerland 384 https:/www.cambridge.org/core/terms. https://doi.org/10.1017/S0317167100032492Downloaded from https:/www.cambridge.org/core. University of Basel Library, on 11 Jul 2017 at 12:04:46, subject to the Cambridge Core terms of use, available atLE JOURNAL CANADIEN DES SCIENCES NEUROLOGIQUES PD 7 8 and in AD.9 1 0 It has also been postulated that the oxida-tive determination of DA by MAO-B with production of hydro-gen peroxide, in the human brain, could be deleterious for the nigrostriatal dopaminergic neurones. Thus, due to the abnormally low content of reduced glutathione and/or in the increased amount of ferrous iron in the substantial nigra of Parkinsonian patients, the hydrogen peroxide formed by deamination of DA might lead to the generation of oxygen free radicals which in turn produce lipid peroxidation, neuronal membrane damage and cell death.1112 In Parkinsonian patients treated with Madopar® or Sinemet® MAO-B inhibitors might decrease the risk of hydroxyl free-radical formation by reducing the formation of hydrogen peroxide generated from the oxidation of DA. According to the free-radical as well as to the neurotoxin hypothesis the MAO-B enzyme might play a pivotal role in the physiopathological processes underlying the so-called "degener-ative" diseases of the aging brain. From Madopar® Standard to Madopar® HBS and the Rationale to Combine MadoparR with the COMT Inhibitor Ro 40-7592 DOPA, the precursor amino acid of the catecholamine (CA) neurotransmitters including DA, administered in combination with peripheral DOPA decarboxylase (DDC) inhibitors, e.g. benserazide (Madopar®) or carbidopa (Simemet®), is still con-sidered the major therapeutic agent for the treatment of PD.13-14 The majority of the problems encountered in the therapy with DOPA originate from its complex pharmacokinetics and phar-macodynamics.15 Being an amino acid, DOPA follows multiple metabolic pathways, and in the gastrointestinal tract as well as at the blood-brain barrier it competes with the transport of a number of large neutral amino acids. Competition for the blood-brain barrier uptake occurs also between DOPA and its O-methylated derivative 3-O-methyl DOPA (3-OMD). 3-OMD is a poor substrate for DDC and because of a much longer half-life (about 15 h) than DOPA (about 1 h) accumulates in the plasma of patients treated with Madopar® and Sinemet®. The ratio of mean plasma 3-OMD to DOPA concentrations can be as large as 14:1 and could lead to clinical deterioration of the patients.15 Elevated 3-OMD concentrations have sometimes been connected to dyskinesias or to on-off phenomena. To reduce the magnitude of fluctuations in plasma DOPA concentrations novel con-trolled-release formulations e.g. Madopar® HBS16or Sinemet® CR415 have been recently introduced. The controlled-release formulations of DOPA plus DDC inhibitors reduce the sudden formation of peak concentrations of DOPA in plasma reducing the number of daily "off periods. The advantage of these formulations is to prolong the mainte-nance of therapeutic DOPA levels in plasma thereby increasing the mobility of the patients even during the night.17 Neurochemical Characteristics of the COMT Inhibitor Ro 40-7592 The nitrocatechol derivative Ro 40-7592 is a competitive (Ki=30 nM) inhibitor of COMT, very active in vitro (1C5Q in rat liver = 36 nM) and in vivo (ED5 0 for rat liver and kidney enzyme inhibition: 2.7 and 1.2 mg/kg p.o., 1 h). Ex vivo, a reli-able index to assess COMT inhibition in cerebral and extracere-bral tissues uses the decreased formation of 3-OMD and homovanillic acid (HVA) in rat brain and plasma.1 Several pre-clinical studies have shown that Ro 40-7592 administered p.o. in combination with benserazide and DOPA almost completely blocks the formation of 3-OMD in rat brain and plasma.118 In these experiments, in addition, a long-lasting increase of DOPA was measured in plasma17 paralleled by a marked and prolonged increased in brain DA.1 In the same experimental condition, a marked decrease of the HVA content was measured in the brain with a parallel remarkable increased of 3,4-dihydroxphenyl-lacetic acid.1 Brain nonadrenaline and H5-hydroxytryptamine were unaffected. In conclusion, by increasing the bioavailability and possibly prolonging the plasma half-life of DOPA as well as by reducing the formation of 3-OMD, Ro 40-7592, combined with peripheral DDC inhibitors and DOPA, e.g. in Madopar® standard or HBS is expected to markedly improve the therapy of PD patients. In healthy subjects Ro 40-7592 at single oral doses was well tolerated up to 800 mg and, at this dose, the compound markedly inhibited (> 80%) human erythrocyte COMT activity for at least 4 h. As already observed in rat, the formation of 3-OMD was virtually suppressed also in human, when Madopar® 125 was co-administered with Ro 40-7592 (200 mg), and DOPA bioavailability was doubled. The available clinical data support the notion that Ro 40-7592 combined with Madopar® standard, and even more so with Madopar®-HBS, will significantly improve DOPA pharmacokinetics (maintaining for several hours optimal therapeutic concentrations of DOPA in plasma by avoiding the formation of sharp peak of DOPA) and prevent for-mation and accumulation of 3-OMD in plasma and brain. It is anticipated that, in the near future, the combination of con-trolled-release formulations of DOPA with Ro 40-7592 will rep-resent an optimal therapeutic regimen by reducing the dose of DOPA and by prolonging interdose intervals. The Novel, Reversible Highly Selective MAO-B Inhibitor Ro 19-6327 To date, selegiline is the only MAO-B inhibitor used clinically as adjunct to Madopar® or Sinemet® to improve symptom fluc-tuations in PD19 and as possible neuroprotective agent in PD7'8 and AD.5.6 However, selegiline at relatively low doses (20 mg daily) and under long-term therapy does not only inhibit MAO-B but also, to some extent, inhibits MAO-A5 with risk of hyper-tensive crises (cheese-effect). Apart from its low specificity, the irreversible MAO-B inhibitor selegiline can be metabolized to potentially neurotoxic derivatives e.g. (-)methamphetamine and (-)amphetamine.20 In contrast to selegiline, Ro 19-6327 belongs to a new chemical class of non-toxic, mechanism-based, fully reversible, and highly selective MAO-B inhibitors both in vitro and ex vivo. Ro 19-6327 is short-lasting, since enzyme activity returned to control values 48 h after dosing even when the com-pound is administered at high doses (100 7imol (23.6 mg)/kg p.o.) to rats. Full reversibility of MAO-B inhibition was con-firmed directly by dialysis experiments.5 Ro 19-6327, which per se did not affect mean arterial blood pressure (MAP), did not potentiate, even at high doses, the tyramine-induced increase in MAP in freely moving rats.5 In black mouse striatal tissue, Ro 19-6327 prevented MPTP-induced depletion of brain DA and the formation of the quaternary derivative MPP+5. Ro 19-6327, due to its highly selective inhibitory effect on MAO-B, is an excellent tool to clinically assess whether complete and selec-tive MAO-B inhibition protects dopaminergic neurones from Volume 18, No. 3 (Supplement) — August 1991 385 https:/www.cambridge.org/core/terms. https://doi.org/10.1017/S0317167100032492Downloaded from https:/www.cambridge.org/core. University of Basel Library, on 11 Jul 2017 at 12:04:46, subject to the Cambridge Core terms of use, available atTHE CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES age-associated degeneration, possibly by reducing the formation of free radicals or the conversion of MPTP-like compounds into neurotoxins in the CNS.1 In contrast to selegiline, Ro 19-6327, even at extremely high doses (350 mg), does not potentiate at all the tyramine pressor effect in healthy subjects. Moreover, this highly selective MAO-B inhibitor, due to its chemical structure cannot be converted into amphetamine-like metabolites.1 In healthy subjects, Ro 19-6327 at relatively low doses (40 mg), completely inhibited platelet MAO-B activity for about 12 h; the compound was well tolerated even at the high dose of 350 mg. By positron emission tomography in man it has been shown that 50 mg Ro 19-6327 markedly (> 90%) inhibited brain MAO-B for at least 12 h. Interestingly, this study showed that the time-course of platelet MAO-B inhibition virtually paralleled the inhibitory effect of Ro 19-6327 on the brain enzyme.21 In con-clusion, the highly selective, specific and non-toxic MAO-B inhibitor Ro 19-6327 provides a novel and safe tool to investi-gate the physiopathological role played by MAO-B in PD and AD and the consequences of its inhibition in the prevention and/or in the therapy of these diseases. REFERENCES 1. Da Prada M, Ziircher G, Kettler R, et al. New therapeutic strategies in Parkinson's disease: inhibition of MAO-B by Ro 19-6327 and of COMT by Ro 40-7592. Adv Behav Biol 1991; 39: 723-732. 2. Glover V, Sandler M, Owen F, et al. Dopamine is a monoamine oxidase B substrate in man. Nature 1977; 265: 80-81. 3. Birkmayer W, Riederer P, Youdim MBH, et al. Potentiation of antikinetic effect after L-dopa treatment by an inhibitor of MAO-B, L-deprenyl J Neural Transm 1975; 36: 303-323. 4. Heikkila RE, Manzino L, Cabbat FS, et al. 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Ro 40-7592, a novel, very potent and orally active inhibitor of catechol-O-methyltrans-ferase: a pharmacological study in rats. Adv Neurol 1990; 53: 497-503. 19. Koller WC, Giron LT. Selegiline HC1: selective MAO-type B inhibitor. Neurology 1990; 40 (Suppl 3): 58-60. 20. Reynolds GP, Elsworth JP, Blank K, et al. Deprenyl is metabolized to methamphetamine and amphetamine in man. Brit J Clin Pharmacol 1978; 6: 542-544. 21. Price GW, Bench CJ, Cremer JC, et al. Inhibition of human brain monoamine oxidase B by Ro 19-6327 - in vivo measurement using positron emission tomography. Eur J Pharmacol 1990; 183: 166. 386 https:/www.cambridge.org/core/terms. https://doi.org/10.1017/S0317167100032492Downloaded from https:/www.cambridge.org/core. University of Basel Library, on 11 Jul 2017 at 12:04:46, subject to the Cambridge Core terms of use, available at

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