Sorbus torminalis (L.) Crantz (Rosaceae), also known as wild service tree, is a
deciduous tree widespread in central and southern Europe, north-western Africa, western
Asia, the Balkan Peninsula and the Caucasus (1, 2). Species of the genus Sorbus represent a
rich source of polyphenols that are responsible for their numerous proven effects, including
anti-diarrhoeal, anti-inflammatory, antidiabetic, antioxidant and anti-atherogenic effect.
Accordingly, the bark of S. torminalis could have interesting composition and effects,
however, data about this herbal drug is insufficient (1). Bark dry methanol extract (DER
9.74:1), obtained after successive extraction with cyclohexane and dichloromethane, was
analyzed for antioxidant potential using DPPH and FRAP assay. Contents of total
polyphenols, flavonoids and tannins were determined using spectrophotometric methods
based on reaction with Folin-Ciocalteu reagent (FC), reaction with aluminum-chloride and
adsorption by hide powder in combination with reaction with FC, respectively. LC-DAD-ESI-
MS analysis was used for identification of main extract constituents. In DPPH test IC50 value
8.66±0.07 μg/mL was shown, while result of FRAP test was 3.01±0.11 μmol Fe 2+ /mg of dry
extract. Contents of total polyphenols, flavonoids and tannins were 0.39±0.06 mg GA/mg of
dry extract, 0.035±0.019% and 1.3±0.08%, respectively. By LC-DAD-ESI-MS analysis 25
compounds were detected among which procyanidins (dimers, trimers and tetramers) are
dominant. The results of this study showed that dry methanol extract of S. torminalis bark is
rich in procyanidins which potentially contribute to its antioxidant activity. Additional
significant effects of this herbal drug and its isolated compounds should be investigated in
future studies.Sorbus torminalis (L.) Crantz (Rosaceae) je listopadno drvo, u narodu poznato pod
nazivom brekinja. Rasprostranjeno je širom centralne i južne Evrope, severozapadne Afrike,
zapadne Azije, Balkanskog poluostrva i Kavkaza (1, 2). Vrste roda Sorbus predstavljaju bogat
izvor polifenola koji su odgovorni za njihove brojne dokazane efekte, uključuju antidijaroični,
antiinflamatorni, antidijabetski, antioksidantni i antiaterogeni efekat. U skladu sa tim, kora S.
torminalis mogla bi imati interesantan sastav i efekte, međutim, o ovoj biljnoj drogi nema
dovoljno podataka (1). Antioksidantna aktivnost suvog metanolnog ekstrakta kore stabla
(DER 9,74:1), dobijenog nakon sukcesivne ekstrakcije cikloheksanom i dihlormetanom,
analizirana je DPPH i FRAP testom. Sadržaji ukupnih polifenola, flavonoida i tanina određeni
su spektrofotometrijskim metodama koje se zasnivaju na reakciji sa Folin‐Ciocalteu
reagensom (FC), reakciji sa aluminijum-hloridom i na adsorpciji na kožni prah u kombinaciji
sa reakcijom sa FC reagensom, redom. LC‐DAD‐ESI‐MS analiza korišćena je za identifikaciju
glavnih sastojaka ekstrakta. U DPPH testu dobijena je IC50 vrednost 8,66±0,07 μg/mL, a
rezultat FRAP testa bio je 3,01±0,11 μmol Fe 2+ /mg suvog ekstrakta. Sadžaj ukupnih
polifenola bio je 0,39±0,06 mg GA/mg suvog ekstrakta, flavonoida 0,035±0,019%, a tanina
1,3±0,08%. LC‐DAD‐ESI‐MS analizom detektovano je 25 jedinjenja od kojih su dominantni
procijanidini (dimeri, trimeri i tetrameri). Rezultati ovog istraživanja pokazali su da je suvi
metanolni ekstrakt kore stabla S. torminalis bogat procijanidinima koji potencijalno
doprinose njegovoj antioksidantnoj aktivnosti. Dodatne značajne efekte ove biljne droge i
njenih izolovanih jedinjenja trebalo bi ispitati u budućim istraživanjima.VIII Kongres farmaceuta Srbije sa međunarodnim učešćem, 12-15.10.2022. Beogra
