Curly dock (Rumex crispus L.) is a perennial herbaceous plant that is traditionally used
in the treatment of gastrointestinal ailments, infections and skin diseases. The aim of this
study was to examine the chemical composition, ex vivo anti-inflammatory effect and in vivo
antiproliferative activity of water extract of curly dock fruit. The polyphenolic profile was
analyzed by LC-DAD-ESI-MS technique. The effect on the production of arachidonic acid
metabolites was examined in human platelets. Antitumor activity of orally administered
extract (concentration: 5% w/v; doses: 1, 2 and 5 mL/kg of body weight/day; treatment
length: 7 days) to Ehrlich ascites tumor and hepatocellular carcinoma was assessed in an
animal experiment. Ascites volume, viable cell percentage and antioxidant enzyme levels
were determined. The presence of phenolic acids (gallic and protocatechuic acids),
proanthocyanidins (monomers, dimers and trimers), flavonoid glycosides and
anthraquinone emodin was revealed in the water extract of R. crispus fruit. The content of
the quantitatively dominant compound miquelianin was 2.4%. The investigated preparation
concentration-dependently inhibited the synthesis of 12(S)-hydroxy-(5Z,8E,10E)-
heptadecatrienoic acid (IC50 = 4.96 mg/mL) and 12(S)-hydroxy-(5Z,8Z,10E,14Z)-
eicosatetraenoic acid (IC 50 = 2.06 mg/mL). The antitumor effect in animals was dose-
dependent and manifested as suppression of tumor growth and induction of cell death of
malignantly transformed cells. Values of biochemical parameters suggested that oxidative
stress was induced in cancer cells, and it can be assumed that this mechanism was at least
partly responsible for the activity. Water extract of R. crispus fruit has significant
pharmacological potential that justifies further research.Štavelj (Rumex crispus L.) je višegodišnja zeljasta biljka koja se tradicionalno koristi u
tretmanu gastrointestinalnih tegoba, infekcija i kožnih oboljenja. Cilj ove studije je bio da se
ispita hemijski sastav, ex vivo antiinflamatorni efekat i in vivo antiproliferativna aktivnost
vodenog ekstrakta ploda štavelja. Polifenolni profil je analiziran LC‐DAD‐ESI‐MS tehnikom.
Uticaj na produkciju metabolita arahidonske kiseline je ispitan u humanim trombocitima.
Antitumorska aktivnost oralno primenjenog ekstrakta (koncentracija: 5% m/V; doze: 1, 2 i 5
mL/kg telesne težine/dan; dužina tretmana: 7 dana) prema Ehrlich-ovom ascitnom tumoru i
hepatocelularnom karcinomu procenjena je u eksperimentu na životinjama. Određeni su
zapremina ascitesa, procenat vijabilnih ćelija i nivoi antioksidantnih enzima. U vodenom
ekstraktu ploda R. crispus je utvrđeno prisustvo fenolnih kiselina (galna i protokatehinska
kiselina), proantocijanidina (monomeri, dimeri i trimeri), flavonoidnih heterozida i
antrahinona emodina. Sadržaj kvantitativno dominantnog jedinjenja mikvelijanina je iznosio
2,4%. Ispitivani preparat je koncentraciono-zavisno inhibirao sintezu 12(S)-hidroksi-
(5Z,8E,10E)-heptadekatrienske kiseline (IC 50 = 4,96 mg/mL) i 12(S)-hidroksi-
(5Z,8Z,10E,14Z)-eikozatetraenske kiseline (IC50 = 2,06 mg/mL). Antitumorski efekat u
životinja je bio dozno-zavisan i ispoljio se kao suprimiranje rasta tumora i indukcija ćelijske
smrti maligno transformisanih ćelija. Vrednosti biohemijskih parametara su sugerisale da je
u ćelijama karcinoma došlo do indukovanja oksidativnog stresa, te se može pretpostaviti da
je ovaj mehanizam bio barem delom zaslužan za aktivnost. Vodeni ekstrakt ploda R. crispus
poseduje značajan farmakološki potencijal koji opravdava dalje istraživanje.VIII Kongres farmaceuta Srbije sa međunarodnim učešćem, 12-15.10.2022. Beogra
