Cinisara is a Sicilian autochthonous breed of dairy cow. Qualitative characteristics of milk yield are influenced by composition of pasture and its changes over the seasons (Di Grigoli et al., 2000). The grazing resource is characterized by notable variability, even in adjacent areas, because of different environmental conditions, such as aspect, the nature of soil and altitude. The aim of this research is to study the effect of season of production on qualitative characteristics of Cinisara cow milk in farms located at different altitudes