AbstractA study was conducted to evaluate Trifolium species for their agronomic traits intwo agro-ecologies of Benishangul-Gumuz region, North Western Ethiopia. Theexperiment was carried out at Tongo, Assosa, and Kamash forage researchstations of Assosa Agricultural Research Center, which were purposively selectedto represent highland, mid and lowland agro-ecologies respectively. FiveTrifolium genotypes (T. steudeneri 9720, T. decorum 9447, T. quantinum 6301, T.tembense 7102, and T. steudeneri 9712) were evaluated in a randomized completeblock design with three replications. Plant height at forage harvesting, forage drymatter yield and leaf to stem ratio were significantly (P < 0.001) affected byenvironment (E) and G x E (G and E interaction). The highest mean forage drymatter yield was recorded at Kamash, than Assosa and Tongo. The result ofcombined analysis indicated that, genotype (G) had significant (P < 0.001) effecton forage dry matter yield and T. quantinum 6301 genotype was the best yielderamong the genotypes evaluated. Total dry matter yield was significantly different(P < 0.001) among genotypes at Tongo and Assosa. Generally, based on foragedry matter yield data, T. quantinum 6301 was well performed at Assosa andTongo than other genotypes, T. steudenary 9720 was relatively better adapted atKamash, and they are recommended as an alternative legume forage crops for therespective locations