BABAZONO Tetsuya

MATSUURA Katsuhisa

MIURA Junnosuke

MOCHIZUKI Shota

SHIMIZU Tatsuya

Tokyo Women’s Medical University

Rat β-Cells Lose Their Proliferative AbilityAfter Birth Through P53 Upregulation著者名 MOCHIZUKI Shota, MATSUURA Katsuhisa, MIURAJunnosuke, SHIMIZU Tatsuya, BABAZONO Tetsuyajournal orpublication titleTokyo Women's Medical University Journalvolume 6page range 91-100year 2022-12-20URL http://hdl.handle.net/10470/00033367doi: 10.24488/twmuj.2022008Antigen Species Cat No. Company DilutionPDX1 Goat AF2419 R&D Systems 1:100Phosphorylated histone H3 Rabbit ab5176 Abcam 1:100Ki67 Rabbit ab15580 Abcam 1:100C-peptide Mouse NBP1-05433 Novus Biologicals 1:100GAPDH Rabbit ab9485 Abcam 1:1000p53 Rabbit bs-0033R Bioss 1:1000Donkey Anti-Goat IgG H&L Alexa Fluor 488Donkey ab150129 Abcam 1:300Donkey Anti-Rabbit IgG H&L Alexa Fluor 594Rabbit ab150076 Abcam 1:300Supplementary Table 1. Antibodies applied for fluorescence immunostaining and western blot. Supplementary Table 2. List of primers used for AppliedBiosystems PCR assay.Gene Applied Biosystems TaqMan assay ID Pak4 Rn01764387_m1Cdkn1b Rn00582195_m1Tp53 Rn007557171_m1ACTB Rn00667869_m1GAPDH Rn01775763_g1(Appendix)　Mochizuki S et al: TWMUJ, 2022Pifithrin-α PDX1 positive cells Number of isletPDX1 positive cells/Number of isletA 10038 43 233B 14995 56 268C 6229 40 156D 6217 37 168E 5051 29 174average 8506 41 200DMSO PDX1 positive cells Number of isletPDX1 positive cells/Number of isletA 2063 18 115B 1287 16 80C 8244 44 187D 5042 26 194E 5275 25 211average 4382 26 158Supplementary Table 3. Number of PDX1-positive cells per islet after 2 weeks of administration of Pifithrin-α

Rat β-Cells Lose Their Proliferative Ability After Birth Through P53 Upregulation

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Rat β-Cells Lose Their Proliferative Ability After Birth Through P53 Upregulation

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