The level structure of Bk249 has been investigated by measuring the γ-ray spectra of an extremely pure Es253 sample obtained by milking this nuclide from Cf253 source material produced in the High Flux Isotope Reactor at Oak Ridge National Laboratory. Additional information on the Bk249 levels was obtained from the β - decay study of Cm249, produced by neutron irradiation of Cm248. Using the results of the present study together with the data from previous Cm248(α,t) and Cm248(He3,d) reactions, the following single-particle states have been identified in Bk249: 7/2+[633], 0.0 keV; 3/2-[521], 8.78 keV; 1/2+[400], 377.55 keV; 5/2+[642], 389.17 keV; 1/2-[530], 569.20 keV; 1/2-[521], 643.0 keV; 5/2-[523], 672.9 keV; and 9/2+[624], 1075.1 keV. Four vibrational bands were identified at 767.9, 932.2, 1150.7, and 1223.0 keV with tentative assignments of {7/2+ [633] 1-}9/2-, {7/2+ [633] 0-}7/2-, {7/2+ [633] 1-}5/2-, and {7/2+ [633] 0+}7/2+, respectively. A band at 899.9 keV was observed in γ-γ coincidence measurements and given a tentative spin assignment of 3/2. It is possibly associated with a 2- phonon coupled to the ground state, with configuration {7/2+ [633] 2-}3/2-. Three levels at 624.3, 703.5, and 769.1 keV were assigned spins of 5/2, 7/2, and 9/2, respectively. These could be the members of the 3/2+ [651] band, expected in this energy region
