In a blind comparison, 465 randomly collected clinical fecal specimens were examined. Hemo-De was found to be an excellent replacement for ethyl acetate in the concentration procedure and for carbol-xylene and xylene in the trichrome staining procedure. Elimination of toxic reagents, combined with its lower cost, makes Hemo-De the preferred choice in routine parasitology examinations

Aldeen, W E

Hale, D

PubMed

In a blind comparison, 465 randomly collected clinical fecal specimens were examined. Hemo-De was found to be an excellent replacement for ethyl acetate in the concentration procedure and for carbol-xylene and xylene in the trichrome staining procedure. Elimination of toxic reagents, combined with its lower cost, makes Hemo-De the preferred choice in routine parasitology examinations. Clinical parasitology has evolved slowly toward elimina-tion of toxic reagents used in parasitologic concentration and trichrome staining procedures. A problem of present-day parasitology is the need to identify suitable cost-effective replacements for ethyl acetate, carbol-xylene, and xylene while maintaining the recovery rate of intestinal parasites. Elimination of these reagents would help to ensure the safety of the technologist performing the examination as well as other laboratory personnel (Table 1). An increasing concern with the use of toxic agents has prompted several studies by Neimeister (5, 6) and Gubas

William E. Aldeenl

Devon Hale

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Use of Hemo-De To Eliminate Toxic Agents Used for Concentration and Trichrome Staining of Intestinal Parasites

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265403

Use of Hemo-De to eliminate toxic agents used for concentration and trichrome staining of intestinal parasites.

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