The heavy metals content (HMs) was investigated in 14 different medicinal plants collected
from the three regions in Central Serbia, Zlatar, Sokobanja, and Kopaonik. The concentrations
of Cd, Cr, Ni, Hg and Pb were determined: Cd (<0.03–2.72 mg/kg); Cr (<0.08–12.1 mg/kg); Ni
(<0.08–12.2 mg/kg); Pb (0.6–49.0 mg/kg); the Hg concentration was below the detection limit of
0.06 mg/kg in all samples. The daily intake of HMs due to ingestion of 200 mL of herbal infusion
was in all cases below the recommended limit prescribed by the World Health Organization. The
estimated daily intake values were below the values for the oral reference dose regulated by the U.S.
Environmental Protection Agency (USA EPA). The target hazard quotient and hazard index for Cd,
Cr Ni, and Pb were below 1. Nevertheless, due to the tendency of heavy metals to accumulate in
the organism, attention should be paid to the daily intake of herbal infusion during long-term usage.
Specifically, it is recommended to consume not more than one cup (200 mL) of infusion per day made
from thyme (Mt. Zlatar) and blueberry (Mt. Kopaonik), and not more than two cups per day for
other herbs