The Editorial Office of Chinese Journal of Food Hygiene
Doi
Abstract
ObjectiveTo develop an analytical method for fast determination of banned pigment quinoline yellow in pastries by salting out assisted-high performance liquid chromatography.MethodsThe sample was extracted with 40% methanol-sodium chloride-water, precipitated with potassium ferrocyanide-zinc acetate solution, eluted with mobile phase of methanol-0.02 mol/L ammonium acetate solution, separated by X-Bridge C18 column v(150 mm×4.6 mm, 3.5 μm), and detected with diode -array detector by external standard method.ResultsThe method showed good linearity (r>0.999) in the range of 0.4-40.0 μg/mL. The limit of detection (S/N=3) was 1.25 mg/kg and the limit of quantification (S/N=10) was 5.0 mg/kg. The average recoveries of three different concentrations level at 5.0, 10.0 and 50.0 mg/kg ranged from 89.18% to 110.10%, with relative standard deviation in the range of 2.83%-8.65%.ConclusionThe method was convenient, accurate and reproducible, and it was suitable for qualitative and quantitative analysis of banned pigment quinoline yellow in pastries