Alsobh Areej

Bánvölgyi Szilvia

Vatai Gyula

English

University of Szeged

28th International Symposium on Analytical and Environmental Problems  72  IDENTIFICATION OF BIOACTIVE COMPOUNDS USING  RP-HPLC ANALYSIS OF SOME HAWTHORN SPECIES EXTRACTS (CRATAEGUS PINNATIFIDA BGE., C. MONOGYNA JACQ., C. CRUS-GALLI.) AND ANTIOXIDANT ACTIVITY EVALUATION. Areej Alsobh1, Gyula Vatai1, Szilvia Bánvölgyi1 1Department of Food Process Engineering, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary e-mail: areejalsobh@gmil.com  Abstract Hawthorn is belonging to the Rosaceae family and is one of the plants that have been used as a source of bioactive products. The goal of this work was to determine the phenolic and flavonoid contents and antioxidant activity of ethanolic extracts of some hawthorn species (Crataegus pinnatifida Bge., C. monogyna Jacq., C. crus-Galli.). The extraction was performed at  45 °C, by using ethanol 50 v/v% as a solvent (10 g of the fruit in 100 ml solvent) for 50 min. The Folin-Ciocalteu’s method was used for the determination of total phenols, the aluminium chloride method was used for the determination of flavonoids, and the ferric reduction antioxidant power (FRAP) method was used to assess the antioxidant activity of extracts. The identification of phenolic compounds present in extracts was performed using RP-HPLC. A positive linear correlation between the antioxidant activity index and total phenolic content of ethanolic extracts was observed. The order of antioxidant activity in species was as follows (C. crus-Galli > C. pinnatifida Bge. > C. monogyna Jacq.)   The RP-HPLC procedure showed that the most abundant compounds were chlorogenic, ferulic and ellagic acids and (+)-Catechin while gallic and caffeic acid were not detected. The extracts have significant antioxidant properties due to the existence of phenolic compounds. It is noteworthy to emphasize that for C. crus-Galli species, its extracts have not been studied or referred to, to the best of our knowledge.  Keywords:  Hawthorn, Total phenolic and flavonoid content, RP-HPLC, Antioxidant Activity   

Identification of bioactive compounds using RP-HPLC analysis of some Hawthorn species extracts (crataegus pinnatifida BGE., C Monogyna Jacq., C. Crus-galli.) and antioxidant activity evaluation : [abstract]

http://acta.bibl.u-szeged.hu/78463/1/proceedings_of_isaep_2022_072.pdf

Identification of bioactive compounds using RP-HPLC analysis of some Hawthorn species extracts (crataegus pinnatifida BGE., C Monogyna Jacq., C. Crus-galli.) and antioxidant activity evaluation : [abstract]

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