del Prete, S.

English

The transition to flowering results from a complex interplay between endogenous and environmental cues. The leaves play a key role in this process, by perceiving the light changes and producing photosynthates, which participate to the floral signalling. However, our knowledge on the changes occurring in leaves during floral transition is still limited. We characterised the morphological, molecular and transcriptional events related to floral transition in mature leaves in Arabidopsis, using a short-day to long-day shift to induce a synchronized flowering. We identified the temporal window of the floral transition, monitored the leaf growth and observed an increase in their ploidy level during the process. By RNA-seq we studied the transcriptional dynamics of the leaf gene network, and compared with events occurring in roots and meristems to get an integrated view of floral transition in the whole plant. Furthermore, we investigated the mode of action of LIKE HETEROPROTEIN 1 (LHP1), a PRC1 subunit, by exploiting transgenic lines with conditional alterations of LHP1 dosage and analysing the effects on chromatin and transcription of flowering genes. A short-term modulation of LHP1 dosage altered the deposition of H3K27me3 and H3K4me3, showing a functional interaction between LHP1 and PRC2, and also suggesting a new role in the formation of bivalent chromatin regions. Finally, since nuclear organisation plays a key role in gene regulation, we searched and identified determinants of the nuclear architecture by using innovative spatial statistical tools

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UvA-DARE is a service provided by the library of the University of Amsterdam (https://dare.uva.nl)UvA-DARE (Digital Academic Repository)Characterisation of transcriptional and chromatin events in relation to floraltransition and identification of nuclear organisation determinantsdel Prete, S.Publication date2017Document VersionOther versionLicenseOtherLink to publicationCitation for published version (APA):del Prete, S. (2017). Characterisation of transcriptional and chromatin events in relation tofloral transition and identification of nuclear organisation determinants. [Thesis, fully internal,Universiteit van Amsterdam, Université Paris Saclay].General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s)and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an opencontent license (like Creative Commons).Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, pleaselet the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the materialinaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letterto: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. Youwill be contacted as soon as possible.Download date:09 Mar 2023 Acknowledgements  This PhD experience taught me about the fascinating word of epigenetics that leave me astounded and the scientific thinking but also a lot about human behaviour. Everyday I get to explore the sensation of climbing a mountain with the excitement and the frustrations, sometimes I was terrible failing and fighting but there has been day in which I reach the summit of the mountain with all the passion that belongs to me since always. Trough this overwhelming experience I reach an important life landmark. Different are the people and friend met along the way, which made my climbing softer and to which I would like to express all my gratitude. First at all I would like to thank my supervisor Valérie for giving me the possibility to be part of an amazing ITN network, for all the shared scientific knowledge and for the efforts to get to the point. Of course also for “ses patisseries et vin françaises”. Merci beaucoup pour tout!! Paul Fransz, for giving me the opportunity to continue and finish the PhD, guesting me in the lab and for the scientific and writing advises. Ronald Koes, for taking care of the UvA bureaucracy and the doctorate regulations and for giving me, with Paul, the possibility to experience the very nice and stimulating UvA working environment. Phillippe Andrey, for the pure moments of learning during the maths/ physics/stat meetings, I really enjoyed. All the bright group leader of the EpiTRAITS network for the discussions that have made the fellows grow day by day. A particular thanks goes to Maike Stam to having led in an excellent way the groups, together with Helen Bergman.  Ortrun Mittelsten Scheid, my amazing mentor for her support during this year and for the fruitful discussion.  All my EpiTRAITS friends, it was such a great time (scientific and social life) with you all. Till my BIG friend, that read my very first draft of chapter 1 and that shared with me his wide scientific knowledge. Suraj my favourite kind indian Prince, that make me know his country. Blaise, for the reading of chapter 5 and pour toute le “force et courage” give to me. Dorota, the sweet blondy girl that shared with me all the room during the meeting and that was making the 260 Acknowledgements busy-meeting days more relaxing with her songs. Pawel, for his spirit of humor even in the most frustrating moments. Javi, my foreigners support (we shared so much together). Dimitrios, for his “Thanks Marie”. Ruri, for her kindness. Jean-Lousi and Johan, the French “combriccola”.  My little INRA-Italian family: Enrico, Elisa, Federica, Dario, Giorgiana per il supporto quotidiano, senza di voi non sarei sopravissuta. Un grazie speciale va a Enrico e Elisa per le spontanee dicsussioni scientifiche fatte insieme. Julien, Corinne, Luc, Trang pour le dejuner a la canteen ensemble et pour tout le colere et rires que nous avons partagé.  Aude e Beatriz for the funny and relaxing time spent together. Noemi, Giacomo e la piccolo patata Ginevra che sono stati un po come la mia famiglia Parigina. Loeiza, Vincent, Matthieu e il loft di Gentilly qui a été ma deuxième maison à Paris. Blaise, Giulia e la bella Matilde per essermi stati vicini nel periodo Olandese. All the very nice people meet at Uva: Ruy, Rachid, Manon, Marc and Anouk that integrate me very fast on the university environment.  Martino, il mio uomo del Norde che mi dona amore quatidiano e con cui é impossibile annoirasi, che ha contribuito alla rilettura di questi tesi e alla sua parte grafica. La mia FAMIGLIA mamma, papa, Luigi che mi é stata sempre vicina e che con i loro sacrifici ha permesso arrivassi a questo traguardo, il loro supporto é stato immensamente grande. Grazie Mille!! Grazie, merci, dank u wel, thank you to all the people that left on me a positive mark and that give me awesome support when needed it! 

Characterisation of transcriptional and chromatin events in relation to floral transition and identification of nuclear organisation determinants

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Characterisation of transcriptional and chromatin events in relation to floral transition and identification of nuclear organisation determinants

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