Brouwer, A.M.

Scherer, T.

van Ramesdonk, H.J.

van Stokkum, I.H.M.

English

2 Lecture conformational changes of intramol. charge-transfer species were studied by means of time resolved fluorescence spectroscopy. From a time resolved fluorescence spectrum the parameters which describe a kinetic, compartmental, model as well as the model dependent fluorescence spectral-shape parameters were estd. Two methods for parameter estn. were compared nonlinear least squares and multiresponse. Anal. of residuals revealed shortcomings of the exptl. setup, in particular time jitter. The concn. fluctuations induced by this time jitter could cause failure of the usual nonlinear least squares model fit, whereas the multiresponse parameter estn. was successful. Different compartmental models with the same kinetic parameters result in identical residuals. Thus a distinction can only be made on the basis of the accompanying estd. spectra. Due to the restriction that fluorescence spectra are nonneg., the anal. of a two-component system was only satisfactory using a model in which a slower decaying component was a reaction product of a faster decaying component. A three-component system was satisfactorily described by a model with three independently decaying components

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UvA-DARE is a service provided by the library of the University of Amsterdam (https://dare.uva.nl)UvA-DARE (Digital Academic Repository)Multiresponse parameter estimation and compartmental analysis of timeresolved fluorescence spectra. Application to conformational dynamics ofcharge-separated species in solutionvan Stokkum, I.H.M.; Brouwer, A.M.; van Ramesdonk, H.J.; Scherer, T.Publication date1993Published inProc.Kon.Ned.Akad.v.Wetensch.Link to publicationCitation for published version (APA):van Stokkum, I. H. M., Brouwer, A. M., van Ramesdonk, H. J., & Scherer, T. (1993).Multiresponse parameter estimation and compartmental analysis of time resolvedfluorescence spectra. Application to conformational dynamics of charge-separated species insolution. Proc.Kon.Ned.Akad.v.Wetensch., 96(1), 43-68.General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s)and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an opencontent license (like Creative Commons).Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, pleaselet the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the materialinaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letterto: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. Youwill be contacted as soon as possible.Download date:09 Mar 2023

Multiresponse parameter estimation and compartmental analysis of time resolved fluorescence spectra. Application to conformational dynamics of charge-separated species in solution

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Multiresponse parameter estimation and compartmental analysis of time resolved fluorescence spectra. Application to conformational dynamics of charge-separated species in solution

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