Bills, Kyle B

Farnsworth, Cole

Henley, Molly

Kay, Walker

Kriak, John A

Manukyan, Varos Victer

Reynolds, Jonathon

Sant, David W

English

eCommons at Roseman University

Determination of Feasibility and Plausibility of Using Various Biological Samples for Remote Genetic TestingCole Farnsworth*, Molly Henley*, Walker Kay, Victer Manukyan, Jonathon Reynolds, John A. Kriak, Kyle B. Bills, David W. Sant1Department of Biomedical Sciences, Noorda College of Osteopathic Medicine, Provo, UT *Authors Contributed EquallyObtaining a biological sample for sequencing at a remote location can be difficult because blood draws require a clinical visit. For this project, we are testing 49 commercially available DNA extraction kits on dried blood spot cards, saliva, buccal swab, and whole blood samples to determine the best combination to obtain high-quality DNA from individuals that cannot travel to a clinic for sample collection.  BackgroundConclusionsMethodsResultsSwab and saliva kits from Mauwi, Zymo, and DNA Genotek have been purchased for testing. Filter paper for DBS collections have been purchased from Qiagen and Cytiva. DNA extracts will be tested for quantity, fragment length, chemical purity, and proportion of DNA obtained that is human.Picture 1. The sample types that will be tested are fried blood spot cards, saliva, swabs, and whole blood. Figure 1. Completion of dried blood spot cards required more lancet punctures than anticipated, with an average of 7 punctures (range 2-14) per card Picture 2. A lancet of this soft is used for obtaining dried blood spot samples. Company Product Cat. NumberBeckman Coulter GenFind V3 Readent Kit-50 C34880Beckman Coulter DNAdvance A48705Biomiga Biomiga EZgene Tissue DNA Kit 50 Preps N/Acytiva life sciences Tissue and cells genomicPrep Mini Spin Kit 28904275cytiva life sciences Nucleon BACC Genomic DNA Extraction Kits RPN8501cytiva life sciences Sera-Xtracta HMW DNA kit 29429140cytiva life sciences Blood genomicPrep Mini Spin Kit 28904264Fortis Life Sciences Pure Tissue DNA Kit EB-TDK-50New England BioLabs Mondarch Genomic DNA Purification kit T3010SNew England BioLabs Monarch HMW DNA Extraction Kit for Blood and Cells T3050SOmega Bio Tek E.Z.N.A. Tissue DNA Kit D3396-01Omega Bio Tek Mag-Bind® Blood & Tissue DNA HDQ 96 Kit M6399-00Omega Bio Tek E.Z.N.A.® Blood DNA Mini Kit D3392-00Omega Bio Tek E.Z.N.A.® SQ Blood DNA Kit D5032-00Perkin Elmer Chemagic DNA Cyte Pure Kit CMG-196Promega Wizard Genomic DNA Purification Kit A1120Promega Wizard SV Genomic DNA Purification System A2360Promega MagaZorb DNA Mini-Prep Kit MB1004Promega ReliaPrep gDNA Tissue Miniprep System A2051Promega Wizard HMW DNA Extraction Kit A2920Promega ReliaPrep™ Blood gDNA Miniprep System A5081Qiagen DNEasy Blood and Tissue Kit 69504Qiagen QIAamp Fast DNA Tissue Kit 51404Qiagen Puregene Tissue Kit 158063Qiagen QIAGEN Genomic-tips 20/G 10223Qiagen MagAttract HMW DNA Kit 67563Qiagen QIAamp DNA Blood Mini Kit 51104Qiagen Blood & Cell Culture DNA Mini Kit (25) 13323Sigma-Aldrich Extract-N-AMP Tissue PCR Kit XNAT2-1KTSigma-Aldrich GenElute Blood Genomic DNA Kit NA2010-1KTTakara NucleoSpin Tissue 740952.5Takara NucleoMag Tissue 744300.1Takara NucleoSpin® 96 DNA RapidLyse 740110.1Takara NucleoBond HMW DNA 740160.2Takara NucleoMag DNA Swab 744601.1Takara NucleoSpin® Blood 740951.5Takara NucleoMag® Blood 200 µL 744501.1Thermo DNA Extract All Reagents Kit 4403319Thermo MagMax DNA Multi-Sample Kit 4413020Thermo JetFlex Genomic DNA Purification Kit A30700Thermo GeneJet Genomic DNA Purification Kit K0721Thermo GeneJet Genomic DNA Purification Kit K0721Thermo PureLink Genomic DNA Mini Kit K182001Thermo PureLink Genomic DNA Mini Kit K182001Thermo ChargeSwitch gDNA Mini Tissue Kit CS11204Zymo Quick-DNA Miniprep D3024Zymo Quick-DNA miniprep plus D4068Zymo Quick-DNA Magbead Plus Kit D4081Zymo Quick-DNA HMW MagBead Kit D6060Figure 3. HbA1c scoresTable 1. Table of kits obtained for DNA extraction from biological samples. Picture 1. Samples that will be tested include saliva, swabs, dried blood spot cards, and buccal swabs.Picture3. A qPCR machine such as this one will be used to test for the presence of PCR inhibitors and microbial DNA.Figure 2. A picture of one of the completed dried blood spot samples on a Qiagen FTA card. 

Determination of Feasibility and Plausibility of Using Various Biological Samples for Remote Genetic Testing

https://ecommons.roseman.edu/cgi/viewcontent.cgi?article=1290&context=researchsymposium

Determination of Feasibility and Plausibility of Using Various Biological Samples for Remote Genetic Testing

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